S Antibody

Datasheet

Code	CSB-PA361129XA01FSF
Size	US$299
Image	Western Blot Positive WB detected in: recombinant protein All lanes: S Antibody at 1:1000 Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 34 kDa Observed band size: 34 kDa
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Target Background

Product Details

Uniprot No.

P03705

Target Names

Alternative Names

Antiholin (Lysis inhibitor S-107) S

Species Reactivity

Escherichia phage lambda (Bacteriophage lambda)

Immunogen

Recombinant Escherichia phage lambda (Bacteriophage lambda) S protein (1-37aa)

Immunogen Species

Escherichia phage lambda (Bacteriophage lambda)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB

Protocols

ELISA Protocol
Western Blotting(WB) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

Polyclonal antibody generation against S protein necessitates sequential immunizations of a rabbit with recombinant Escherichia phage lambda (Bacteriophage lambda) folA protein (1-37aa). Once an effective antibody titer is achieved, the rabbit is bled, and antibodies are purified from the serum through affinity chromatography. The resulting folA antibody is then tested for functionality in ELISA and WB applications, displaying reactivity with Escherichia phage lambda (Bacteriophage lambda) folA protein.

The S protein of Escherichia phage lambda plays a crucial role in the infection process. It mainly mediates the attachment of the phage to the host bacterium and facilitates the injection of its genetic material into the host cell. It accomplishes this by causing structural changes in the tail of the phage, which then punctures the bacterial cell wall and delivers the viral genetic material. The S protein also helps in stabilizing the interaction between the phage and the host cell during the injection process.

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Target Background

Function

Accumulates harmlessly in the cytoplasmic membrane until it reaches a critical concentration that triggers the formation of micron-scale pores (holes) causing host cell membrane disruption and endolysin escape into the periplasmic space. Determines the precise timing of host cell lysis. Participates with the endolysin and spanin proteins in the sequential events which lead to the programmed host cell lysis releasing the mature viral particles from the host cell.; Counteracts the aggregation of the holin molecules and thus of pore formation.

Subcellular Location

Host cell inner membrane; Multi-pass membrane protein.

Protein Families

Lambdalikevirus holin family

Database Links

KEGG: vg:2703479

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