ICSA Antibody

Code CSB-PA745216XA01SZB
Size US$299
Image
  • Western Blot
    Positive WB detected in Recombinant protein
    All lanes: ICSA antibody at 1:2000
    Secondary
    Goat polyclonal to rabbit IgG at 1/50000 dilution
    Predicted band size: 40.3 kDa
    Observed band size: 45 kDa
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Product Details

Uniprot No.
Target Names
ICSA
Alternative Names
Outer membrane protein IcsA autotransporter [Cleaved into: Outer membrane protein IcsA; Outer membrane protein IcsA translocator] icsA virG CP0182
Species Reactivity
Shigella flexneri
Immunogen
Recombinant Shigella flexneri ICSA protein (311-547aa)
Immunogen Species
Shigella flexneri
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Protein G
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time
Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

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Target Background

Function
Essential for bacterial spreading by eliciting polar deposition of filamentous actin (actin-based motility). Inside the host cell mediates nucleation and polymerization of actin molecules on the bacterial surface, which provides the propulsive force for intracellular movement and intercellular dissemination of the bacterium. During invasion of mammalian cells, triggers autophagy by binding to APG5L. Interaction with IcsB leads to escape from the autophagic host defense system. Also binds ATP and displays weak ATPase activity.
Gene References into Functions
  1. Identification of the H-NS and VirF-binding sites on the icsA promoter regions. PMID: 21724612
  2. Data identified a putative autochaperone region required for IcsA biogenesis, and our data suggest an additional region, not previously identified, is required for N-WASP recruitment. PMID: 18456802
Subcellular Location
[Outer membrane protein IcsA autotransporter]: Periplasm.; [Outer membrane protein IcsA]: Secreted. Cell surface. Note=The alpha domain is localized as a cap over the old pole of the bacterial surface.; [Outer membrane protein IcsA translocator]: Cell outer membrane; Multi-pass membrane protein. Note=The cleaved C-terminal fragment (autotransporter domain) is localized in the outer membrane.
Database Links

KEGG: sfl:CP0182

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