Jak3 Antibody

Code CSB-PA717252ZA01MO
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Product Details

Full Product Name Rabbit anti-Mus musculus (Mouse) Jak3 Polyclonal antibody
Uniprot No. Q62137
Target Names Jak3
Alternative Names Jak3 antibody; Tyrosine-protein kinase JAK3 antibody; EC 2.7.10.2 antibody; Janus kinase 3 antibody; JAK-3 antibody
Raised in Rabbit
Species Reactivity Mus musculus
Immunogen Recombinant Mus musculus Jak3 protein
Immunogen Species Mus musculus (Mouse)
Conjugate Non-conjugated
Clonality Polyclonal
Isotype IgG
Purification Method Protein A/G
Concentration It differs from different batches. Please contact us to confirm it.
Buffer Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form Liquid
Tested Applications ELISA, WB (ensure identification of antigen)
Protocols ELISA Protocol
Troubleshooting and FAQs Antibody FAQs
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables ① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee ① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time Made-to-order (12-14 weeks)

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Target Background

Function
(From Uniprot)
Non-receptor tyrosine kinase involved in various processes such as cell growth, development, or differentiation. Mediates essential signaling events in both innate and adaptive immunity and plays a crucial role in hematopoiesis during T-cells development. In the cytoplasm, plays a pivotal role in signal transduction via its association with type I receptors sharing the common subunit gamma such as IL2R, IL4R, IL7R, IL9R, IL15R and IL21R. Following ligand binding to cell surface receptors, phosphorylates specific tyrosine residues on the cytoplasmic tails of the receptor, creating docking sites for STATs proteins. Subsequently, phosphorylates the STATs proteins once they are recruited to the receptor. Phosphorylated STATs then form homodimer or heterodimers and translocate to the nucleus to activate gene transcription. For example, upon IL2R activation by IL2, JAK1 and JAK3 molecules bind to IL2R beta (IL2RB) and gamma chain (IL2RG) subunits inducing the tyrosine phosphorylation of both receptor subunits on their cytoplasmic domain. Then, STAT5A AND STAT5B are recruited, phosphorylated and activated by JAK1 and JAK3. Once activated, dimerized STAT5 translocates to the nucleus and promotes the transcription of specific target genes in a cytokine-specific fashion.
Gene References into Functions
  1. Study investigated the effect of Jak3 signaling on differentiation from nestin progenitor cells using E13.5 spinal progenitor cell cultures. Results indicated that neuronal and microglial cell differentiation was regulated primarily by Jak3 signaling and the developing neurons and neurite outgrowth might also be regulated by Jak3-dependent microglial activity. PMID: 28817455
  2. JAK3 up-regulates SGLT1 activity by increasing the carrier protein abundance in the cell membrane, an effect enforcing cellular glucose uptake into activated lymphocytes and thus contributing to the immune response. PMID: 27595398
  3. Foxp3 has a rapid turn over in Treg partly controlled at the transcriptional level by the JAK/STAT pathway PMID: 27077371
  4. JAK3 contributes to the regulation of membrane Kv1.5 protein abundance and activity, an effect sensitive to ouabain and thus possibly involving Na(+)/K(+) ATPase activity. PMID: 26100849
  5. JAK3 deficiency is followed by down-regulation of cytosolic Ca(2+) release, receptor and store operated Ca(2+) entry and Na(+)/Ca(2+) exchanger activity in dendritic cells. PMID: 26279433
  6. Jak3 has a role in promoting mucosal tolerance through suppressed expression and limiting activation of TLRs thereby preventing intestinal and systemic chronic low-grade inflammation and associated obesity and MetS PMID: 26451047
  7. Our results demonstrate that JAK3/STAT6 has an important role in bone marrow-derived fibroblast activation, extracellular matrix production, and interstitial fibrosis development. PMID: 26032813
  8. results showed that different JAK3 mutations induce constitutive activation through distinct mechanisms, pointing to specific therapeutic perspectives PMID: 26446793
  9. JAK3 down-regulates Na(+)/K(+)-ATPase activity, an effect involving gene expression and profoundly curtailing ATP consumption. PMID: 26021261
  10. In conclusion, JAK3 deficiency leads to increased formation of calcitriol, which contributes to or even accounts for increased release of FGF23 and enhanced intestinal phosphate absorption. PMID: 25493954
  11. JAK3 deficiency leads to intestinal sodium (Na+) loss, decrease of blood pressure, increased aldosterone release and subsequent stimulation of renal tubular Na(+) reabsorption. PMID: 24281140
  12. JAK3 is a powerful regulator of the peptide transporters PEPT1 and PEPT2. PMID: 23934551
  13. cPLA2, p38 mitogen-activated protein kinase (MAPK), and Janus kinase 3 (JAK3) signaling are necessary for F. tularensis-induced PGE2 production. PMID: 24343645
  14. the essential role of Jak3 in the colon where it facilitated mucosal differentiation by promoting the expression of differentiation markers and enhanced colonic barrier functions through AJ localization of beta-catenin. PMID: 24045942
  15. The potential effects of pharmacological JAK3 inhibition in a myocardial ischemia and reperfusion injury model, were investigated. PMID: 23680658
  16. Inhibition of JAK3 suppressed phosphorylation of PI3K downstream effectors including Akt, mammalian target of rapamycin complex 1, glycogen synthase kinase 3beta, and CREB. JAK3 differentially regulates TLR-mediated inflammatory cytokine production. PMID: 23797672
  17. hemagglutinin of H5N1 avian influenza triggered an abnormal innate immune signalling in the pulmonary epithelial cells, through an unusual process involving activation of Janus kinase 3 PMID: 22359619
  18. analysis of the biochemistry, immunological functions, and clinical significance of JAK3 [review] PMID: 22130498
  19. These findings highlight the pathological role of CD8(+) T cells and Jak3-dependent IFN-gamma-mediated Th1 responses in cerebral malaria pathogenesis. PMID: 22363534
  20. JAK3 inhibition potently facilitates myoblast differentiation through antagonistic STAT1/STAT3 activities. PMID: 22120524
  21. dominant-negative mutants of either Cullin1 or Cullin5, which lack the C terminus responsible for recruiting the E2 enzymes, were able to prevent JAK3 degradation induced by both ASB2/SKP2 and NOTCH signaling. PMID: 21969365
  22. This study defines a novel JAK3 mutation among patients with N-DS AML and demonstrates that normal individuals can also display germline JAK3 substitutions, previously proven to have oncogenic properties, in vitro and in vivo. PMID: 21599579
  23. Janus kinase 3 is expressed in erythrocytes, phosphorylated upon energy depletion and involved in the regulation of suicidal erythrocyte death. PMID: 21691072
  24. Human cord blood-derived CD34+ hematopoietic stem cells were successfully engrafted into Balb/c Rag-2-/-Jak3-/- mice; however, the engraftment rate was far lower in C57/BL6 Rag-2-/-Jak3-/- mice. PMID: 21331358
  25. Jak3 inhibition induced neuronal differentiation accompanied by neurite growth. PMID: 20979137
  26. the CD69 cytoplasmic tail associates with the Jak3/Stat5 signaling pathway, which regulates the transcription of RORgammat and, consequently, differentiation toward the Th17 lineage PMID: 20696842
  27. despite its dependence on leukemia inhibitory factor, JAK3 is not essential for LIF-mediated photoreceptor protection or gene expression. PMID: 20345762
  28. Jak3 is important for dendritic cell maturation, migration and function, through a CCR7-mediated signalling pathway PMID: 19759904
  29. Nonhematopoietic expression of Janus kinase 3 is required for eosinophilic airway inflammation PMID: 11859141
  30. plays several important roles in B cells: during development, to enable cell division, Ig gene rearrangement and cell differentiation, and in mature cells, to promote B cell survival in the periphery PMID: 12147626
  31. Activation of this pathway is associated with granulocytic differentiation of myeloid precursor cells PMID: 12351382
  32. observations establish JAK3 as a key mediator of severe GVHD after allogeneic BMT in the context of a major-HLA disparity PMID: 12389628
  33. Jak3 is a primary response gene for interleukin-6 in macrophage differentiation, and ectopic overexpression of Jak3 accelerates monocytic differentiation of normal mouse bone marrow cells stimulated with cytokines PMID: 14976041
  34. Jak3 is not essential for dendritic cell development but unexpectedly appears to be an important negative regulator PMID: 16020505
  35. the effect of IL-15 and IL-21, which are closely related to IL-2 and share the usage of the common gamma chain and of its JAK3-associated pathway. PMID: 17938255
  36. PDGFRalpha and JAK3 have roles in Galpha(12)-mediated cell proliferation PMID: 18038259
  37. The efficacy observed with CP-690550 in collagen-induced arthritis and adjuvant arthritis suggests JAK3 inhibition may represent novel therapeutic target for treatment of rheumatoid arthritis. PMID: 18234077
  38. data indicate that Janus-kinase-3 regulates epigenetic modification and chromatin remodeling of the Ifng locus during Th1 cell differentiation PMID: 18549798
  39. somatic loss of BLNK and concomitant mutations leading to constitutive activation of Jak3/STAT5 pathway result in the generation of pre-B-cell leukemia PMID: 19047679
  40. functions of JAK3 in the development of skin immune responses and diseases such as psoriasis PMID: 19596999

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Subcellular Location Endomembrane system; Peripheral membrane protein. Cytoplasm.
Protein Families Protein kinase superfamily, Tyr protein kinase family, JAK subfamily
Tissue Specificity In contrast with the ubiquitous expression of the other JAKs, JAK3 is predominantly expressed in hematopoietic tissues.
Database Links

KEGG: mmu:16453

STRING: 10090.ENSMUSP00000060073

UniGene: Mm.249645

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