dgkA Antibody

Code CSB-PA245560XA01ENV
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Product Details

Full Product Name
Rabbit anti-Escherichia coli (strain K12) dgkA Polyclonal antibody
Uniprot No.
Target Names
dgkA
Alternative Names
dgkA antibody; b4042 antibody; JW4002Diacylglycerol kinase antibody; DAGK antibody; EC 2.7.1.107 antibody; Diglyceride kinase antibody; DGK antibody
Raised in
Rabbit
Species Reactivity
Escherichia coli (strain K12)
Immunogen
Recombinant Escherichia coli (strain K12) dgkA protein
Immunogen Species
Escherichia coli (strain K12)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Catalyzes the ATP-dependent phosphorylation of sn-l,2-diacylglycerol (DAG) to phosphatidic acid. Involved in the recycling of diacylglycerol produced as a by-product during membrane-derived oligosaccharide (MDO) biosynthesis. In vitro, phosphorylates various substrates, including sn-1,2-dioleoylglycerol, sn-1,2-dioctanoylglycerol, sn-1,2-dipalmitoylglycerol, sn-1,2-dipalmitate and ceramide. Catalyzes direct phosphoryl transfer from Mg-ATP to diacylglycerol and does not form an enzyme-phosphate intermediate.
Gene References into Functions
  1. The active site of dgkA including glutamates and an aspartate plays roles in substrate binding and catalysis. PMID: 26673816
  2. Magic angle spinning NMR allows simultaneous & direct detection of both ATP hydrolysis and diacylglycerol phosphorylation for DGK; data yield conclusions about intersubunit cooperativity, reaction stoichiometries and phosphoryl transfer mechanism. PMID: 21423170
  3. Results describe the mechanism of folding and insertion into lipid bilayers of Escherichia coli diacylglycerol kinase from a partially denatured state in urea. PMID: 15544815
  4. Diacylglycerol kinase from Escherichia coli contains three transmembrane alpha-helices per monomer. We used fluorescence techniques to define region of first transmembrane helix that spans the hydrophobic core of the reconstituted membranes. PMID: 17722884
  5. determination of the three-dimensional structure of the diacylglycerol kinase homotrimer with the use of solution nuclear magnetic resonance PMID: 19556511

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Subcellular Location
Cell inner membrane; Multi-pass membrane protein.
Protein Families
Bacterial diacylglycerol kinase family
Database Links
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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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