AAA1 Antibody

Code CSB-PA874400XA01DOA
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Product Details

Full Product Name
Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) AAA1 Polyclonal antibody
Uniprot No.
Target Names
AAA1
Alternative Names
AAA1 antibody; BOT1 antibody; ERH3 antibody; FRA2 antibody; FTR antibody; KSS antibody; KTN1 antibody; LUE1 antibody; At1g80350 antibody; F5I6.10Katanin p60 ATPase-containing subunit A1 antibody; Katanin p60 subunit A1 antibody; EC 5.6.1.1 antibody; CAD ATPase antibody; Katanin-1 antibody; Atp60 antibody; Protein BOTERO 1 antibody; Protein ECTOPIC ROOT HAIR 3 antibody; Protein FAT ROOT antibody; Protein FRAGILE FIBER 2 antibody; AtAAA1 antibody; p60 katanin antibody
Raised in
Rabbit
Species Reactivity
Arabidopsis thaliana
Immunogen
Recombinant Arabidopsis thaliana AAA1 protein
Immunogen Species
Arabidopsis thaliana (Mouse-ear cress)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Severs microtubules in vitro in an ATP-dependent manner. Required for oligomerization of functional KTN80-KTN1 complexes that catalyze microtubule severing. This activity may promote rapid reorganization of cellular microtubule arrays. May be required for reorientation of cortical microtubule arrays during cellular elongation. Failure to correctly orient these arrays drastically compromises fiber length, cell wall thickness and mechanical strength. May also be required for the spatial organization of developmental cues within the root.
Gene References into Functions
  1. the proteome-wide effects in two KATANIN 1 mutants, are reported. PMID: 28706004
  2. loss of KTN1 prevented random microtubule networks from shifting into well-ordered arrays. We further showed that the filamentous actin cap, which is a typical feature of several plant epidermal cell types including root hairs and leaf trichomes, was not observed in the growth apexes of conical cells during cell development. PMID: 28644898
  3. Severing at cortical microtubule crossover sites was completely abolished in the Arabidopsis katanin mutant. Consistent with this finding, GFP-tagged katanin driven by its native promoter localizes at sites of CMT crossover prior to severing. PMID: 24206847
  4. study discovered that microtubule severing by the protein katanin plays a crucial and unexpected role in the reorientation of cortical arrays, as triggered by blue light PMID: 24200811
  5. Study shows that the microtubule-severing protein katanin is required to orchestrate growth between neighboring cells by providing cells with the ability to respond efficiently to mechanical stress. PMID: 22500806
  6. The role of katanin, the only known plant microtubule-severing protein, in the organization of cortical microtubules in A. thaliana is reported. PMID: 16805733

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Subcellular Location
Cytoplasm, cytoskeleton.
Protein Families
AAA ATPase family, Katanin p60 subunit A1 subfamily
Tissue Specificity
Expressed ubiquitously, including siliques, flowers, leaves, stems and roots.
Database Links

KEGG: ath:AT1G80350

STRING: 3702.AT1G80350.1

UniGene: At.11879

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