lacI Antibody

1. Effect of helix length on the stability of the Lac repressor antiparallel coiled coil. PMID: 25969365
2. LacI autoregulation balances two opposing states, one that allows quicker response to smaller pulses of external lactose, and the other that minimizes production costs in the absence of lactose. PMID: 23658223
3. Data indicate that in the absence of its inducer, LacI binds to primary operator lac O1 near the transcription start site of the lac operon, and represses its transcription. PMID: 22594825
4. findings show the lac repressor slides 45 +/- 10 base pairs on chromosomal DNA and that sliding can be obstructed by other DNA-bound proteins near the operator; the repressor frequently slides over its natural lacO(1) operator several times before binding PMID: 22723426
5. The role of lactose repressor residues asparagine-125 and aspartate-149 illustrate how readily the ligand binding and allosteric response function of a regulatory bacterial protein can be altered, generating diminished operator binding. PMID: 21928765
6. Using the extensive LacI family of transcriptional factors, identified a set of specificity correlations--determined by two AAs of the TFs and two NTs in the binding sites- and that act as a relatively consistent recognition code. PMID: 21085639
7. solution structure and dynamics of the complex of a dimeric lac repressor DNA binding domain with nonspecific DNA PMID: 15256668
8. The Lac repressor artificially attached to the Escherichia coli cytoplasmic membrane is able to perfectly represses transcription initiated at the tac operator-promoter present on a plasmid and even in the chromosome. PMID: 15867195
9. Linear DNA segments of short-to-medium chain length (50-180 bp) give rise to loops with the extended form of LacR and loops formed within negatively supercoiled plasmids induce the V-shaped structure. PMID: 16785444
10. The mutational spectrum of the genomic lacI gene induced by low-energy nitrogen ion irradiation in wild type Escherichia coli strain W3110 were compared with the spontaneous and the vacuum controls. PMID: 17049362
11. In single molecule experiments, both specific bending and non-specific compaction by integration host factor alter LacI-mediated looping of DNA PMID: 17543441
12. The altered hydrogen bonding can account in part for the different structural conformations of the repressor, and is vital for the allosteric transition PMID: 17543986
13. The structure of lactose repressor (LacI) protein confirms previously reported structures on the monomer level, however, the tetramer is much more densely packed. PMID: 19004002
14. irradiation induces the change of the oligomerization state of LacI from tetramer to dimer. PMID: 19520056

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KEGG: ecj:JW0336

STRING: 316385.ECDH10B_1357