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LAMB Antibody

Rare Species
Datasheet
Code CSB-PA365810XA01ENV
Size US$299
Image
  • Western Blot
    Positive WB detected in Recombinant protein
    All lanes: LAMB antibody at 1:2000
    Secondary
    Goat polyclonal to rabbit IgG at 1/50000 dilution
    Predicted band size: 29.8 kDa
    Observed band size: 31 kDa
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Product Details

Full Product Name
Rabbit anti-Escherichia coli (strain K12) lamB Polyclonal antibody
Uniprot No.
P02943
Target Names
lamB
Alternative Names
lamB antibody; malB antibody; b4036 antibody; JW3996 antibody; Maltoporin antibody; Lambda receptor protein antibody; Maltose-inducible porin antibody
Raised in
Rabbit
Species Reactivity
Escherichia coli (strain K12)
Immunogen
Recombinant Escherichia coli (strain K12) LAMB protein
Immunogen Species
Escherichia coli (strain K12)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Protein A/G
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB
Protocols
ELISA Protocol
Western Blotting(WB) Protocol
Troubleshooting and FAQs
Antibody FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time
Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Description

The production of the LAMB polyclonal antibody involves introducing recombinant Escherichia coli (strain K12) LAMB protein into a rabbit, which triggers the production of antibodies that specifically target this antigen. Antibody production is further amplified through a booster injection, and the resulting LAMB polyclonal antibodies in the rabbit's serum are collected and purified using protein A/G. Rigorous testing conducted in ELISA and WB assays has conclusively demonstrated the LAMB antibody's precise ability to recognize Escherichia coli (strain K12) LAMB protein.

The Escherichia coli (strain K12) LAMB protein serves as a component of the bacterial outer membrane. It is involved in maintaining the structural integrity of the outer membrane and plays a role in the transport of molecules across the membrane. Additionally, the LAMB protein is also known to be involved in bacterial adhesion and biofilm formation.

Usage
For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Citations

Related Products

lamB Proteins

Customer Reviews and Q&A

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Target Background

Function
Involved in the transport of maltose and maltodextrins. Indispensable for translocation of maltodextrins (alpha 1-4 linked polyglucosyls) containing more than three glucosyl moieties. A hydrophobic path ('greasy slide') of aromatic residues serves to guide and select the sugars for transport through the channel. Also acts as a receptor for several bacteriophages including lambda. Binds maltosaccharides; when LamB binds starch in soft agar, it inhibits motility.
Gene References into Functions
  1. Our data indicate that the negative regulation by LamB is widely detected in antibiotic-resistant E. coli. LamB serves as a porin to influx an antibiotic and is interacted with Odp1. PMID: 24412198
  2. Results report the crystallization of E. coli maltoporin in a new crystal form; the crystals belonged to the trigonal R3 space group and diffracted to 1.9 A resolution. PMID: 21206039
  3. downregulation of LamB expression in the CTC-resistant E. coli strain and the increased antibiotic resistance in its gene-deleted strain suggested a negative regulation mechanism in E. coli in response to CTC PMID: 20718490
  4. demonstrated that LamB moves along the bacterial surface and that these movements are restricted by an underlying dynamic spiral pattern PMID: 15341654
  5. all aromatic residues provide some steric hindrance for ion transport through LamB PMID: 16095613

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Subcellular Location
Cell outer membrane; Multi-pass membrane protein.
Protein Families
Porin LamB (TC 1.B.3) family
Database Links
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