LIMK1 Antibody

Code CSB-PA012952XA01DLU
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Product Details

Full Product Name
Rabbit anti-Drosophila melanogaster (Fruit fly) LIMK1 Polyclonal antibody
Uniprot No.
Target Names
LIMK1
Alternative Names
LIMK1 antibody; LIMK antibody; CG1848LIM domain kinase 1 antibody; LIMK-1 antibody; EC 2.7.11.1 antibody; dLIMK antibody
Raised in
Rabbit
Species Reactivity
Drosophila melanogaster (Fruit fly)
Immunogen
Recombinant Drosophila melanogaster (Fruit fly) LIMK1 protein
Immunogen Species
Drosophila melanogaster (Fruit fly)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Protein kinase which regulates actin filament dynamics. Phosphorylates and inactivates the actin binding/depolymerizing factor tsr/cofilin, thereby stabilizing the actin cytoskeleton. Modulation of actin cytoskeleton dynamics may be essential for imaginal disk morphogenesis and axon guidance.
Gene References into Functions
  1. Genetic analysis in Drosophila midgut revealed a conserved role for dLIMK in constraining gastrointestinal stem cell proliferation. PMID: 23585469
  2. functional connections at the behavioral level (learning/memory) depending on the GDNF and LIMK1 brain expression and HSPs transduction that might provide targets for complex approaches for the neurodegenerative diseases treatment. PMID: 23401971
  3. Rac may signal through Pak and LIMK to result in uniform phosphorylation of cofilin in all the outer border cells. PMID: 21205790
  4. LIMK1 localizes near synaptic microtubules, highlighting a novel requirement for LIMK1 during synapse stabilization PMID: 16129399
  5. Limk is a critical regulator of cofilin function and synapse development, and a downstream effector of p21-activated kinase (Pak) in vivo. PMID: 16529736
  6. The higher resistance of characterictics of behavior and communicative sound signals to heat shock is in agreement with the fact that the extremely high LIMK1 and P-cofilin concentrations in cells go down to normal values after heat shock. PMID: 19435260

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Subcellular Location
Cytoplasm. Cleavage furrow. Midbody. Note=Localizes to the cleavage furrow and the midbody at cytokinesis.
Protein Families
Protein kinase superfamily, TKL Ser/Thr protein kinase family
Tissue Specificity
Expressed throughout the imaginal disks of the eye, leg and wing.
Database Links

KEGG: dme:Dmel_CG1848

STRING: 7227.FBpp0073493

UniGene: Dm.3774

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