Recombinant Drosophila melanogaster LIM domain kinase 1 (LIMK1), partial

Code CSB-YP012952DLU
MSDS
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Source Yeast
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Code CSB-EP012952DLU
MSDS
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Source E.coli
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Code CSB-EP012952DLU-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP012952DLU
MSDS
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Source Baculovirus
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Code CSB-MP012952DLU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
LIMK1
Uniprot No.
Alternative Names
LIMK1; LIMK; CG1848LIM domain kinase 1; LIMK-1; EC 2.7.11.1; dLIMK
Species
Drosophila melanogaster (Fruit fly)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Protein kinase which regulates actin filament dynamics. Phosphorylates and inactivates the actin binding/depolymerizing factor tsr/cofilin, thereby stabilizing the actin cytoskeleton. Modulation of actin cytoskeleton dynamics may be essential for imaginal disk morphogenesis and axon guidance.
Gene References into Functions
  1. Genetic analysis in Drosophila midgut revealed a conserved role for dLIMK in constraining gastrointestinal stem cell proliferation. PMID: 23585469
  2. functional connections at the behavioral level (learning/memory) depending on the GDNF and LIMK1 brain expression and HSPs transduction that might provide targets for complex approaches for the neurodegenerative diseases treatment. PMID: 23401971
  3. Rac may signal through Pak and LIMK to result in uniform phosphorylation of cofilin in all the outer border cells. PMID: 21205790
  4. LIMK1 localizes near synaptic microtubules, highlighting a novel requirement for LIMK1 during synapse stabilization PMID: 16129399
  5. Limk is a critical regulator of cofilin function and synapse development, and a downstream effector of p21-activated kinase (Pak) in vivo. PMID: 16529736
  6. The higher resistance of characterictics of behavior and communicative sound signals to heat shock is in agreement with the fact that the extremely high LIMK1 and P-cofilin concentrations in cells go down to normal values after heat shock. PMID: 19435260

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Subcellular Location
Cytoplasm. Cleavage furrow. Midbody. Note=Localizes to the cleavage furrow and the midbody at cytokinesis.
Protein Families
Protein kinase superfamily, TKL Ser/Thr protein kinase family
Tissue Specificity
Expressed throughout the imaginal disks of the eye, leg and wing.
Database Links

KEGG: dme:Dmel_CG1848

STRING: 7227.FBpp0073493

UniGene: Dm.3774

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