lsrK Antibody

Code CSB-PA303534XA01ENV
Size Enquire
Have Questions? Leave a Message or Start an on-line Chat

Product Details

Full Product Name
Rabbit anti-Escherichia coli (strain K12) lsrK Polyclonal antibody
Uniprot No.
Target Names
lsrK
Alternative Names
lsrK antibody; ydeV antibody; b1511 antibody; JW1504 antibody; Autoinducer-2 kinase antibody; AI-2 kinase antibody; EC 2.7.1.189 antibody
Raised in
Rabbit
Species Reactivity
Escherichia coli (strain K12)
Immunogen
Recombinant Escherichia coli (strain K12) lsrK protein
Immunogen Species
Escherichia coli (strain K12)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

Customer Reviews and Q&A

 Customer Reviews

There are currently no reviews for this product.

Submit a Review here

Target Background

Function
Catalyzes the phosphorylation of autoinducer-2 (AI-2) to phospho-AI-2, which subsequently inactivates the transcriptional regulator LsrR and leads to the transcription of the lsr operon. Phosphorylates the ring-open form of (S)-4,5-dihydroxypentane-2,3-dione (DPD), which is the precursor to all AI-2 signaling molecules, at the C5 position. Required for the regulation of the lsr operon and many other genes.
Gene References into Functions
  1. LsrK was expressed and purified from E. coli with linker tags containing tyrosine or glutamine residues, attached to a chitosan film and a capsule, and used in quorum sensing studies. PMID: 28782813
  2. The soluble expression and crystallization of the Escherichia coli quorum-sensing protein LsrK has been described. PMID: 28471356
  3. These results suggest the involvement of AI-2-mediated quorum sensing in K88(+) Enterotoxigenic Escherichia coli PMID: 21300771
  4. AI-2-mediated regulation of enterohemorrhagic Escherichia coli chemotaxis and colonization, as well as temporal regulation of EHEC transcriptome by AI-2. PMID: 18256823
  5. Culture supernatants of E. coli and Staphylococcus aureus luxS mutants were found not to contain detectable levels of AI-2 activity. PMID: 18564424

Show More

Hide All

Subcellular Location
Cytoplasm.
Protein Families
FGGY kinase family
Database Links
icon of phone
Call us
301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
icon of address
Address
7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
icon of social media
Join us with

Subscribe newsletter

Leave a message

* To protect against spam, please pass the CAPTCHA test below.
CAPTCHA verification
© 2007-2024 CUSABIO TECHNOLOGY LLC All rights reserved. 鄂ICP备15011166号-1