MAN7 Antibody

Code CSB-PA861819XA01DOA
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Product Details

Full Product Name
Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) MAN7 Polyclonal antibody
Uniprot No.
Target Names
MAN7
Alternative Names
MAN7 antibody; At5g66460 antibody; K1F13.12Mannan endo-1,4-beta-mannosidase 7 antibody; EC 3.2.1.78 antibody; Beta-mannanase 7 antibody; Endo-beta-1,4-mannanase 7 antibody; AtMAN7 antibody
Raised in
Rabbit
Species Reactivity
Arabidopsis thaliana
Immunogen
Recombinant Arabidopsis thaliana MAN7 protein
Immunogen Species
Arabidopsis thaliana (Mouse-ear cress)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Required for both, loosening of the micropylar endosperm, and rupture of the seed coat in germinating seeds. May participate in the hydrolysis of the mannans in the cell wall of germinating seeds.
Gene References into Functions
  1. Data indicate that the expression of AtMAN7 gene (At5g66460) gene drastically reduced in the knock-out lines of AtbZIP44 (At1g75390). PMID: 23461773
  2. AtMAN7, AtMAN5 and specially AtMAN6 are important for the germination of A. thaliana seeds by facilitating the hydrolysis of the mannan-rich endosperm cell walls. [AtMAN7] PMID: 20878180
Subcellular Location
Secreted.
Protein Families
Glycosyl hydrolase 5 (cellulase A) family
Tissue Specificity
Expressed in stems, flowers, siliques and seeds. Expressed in root vasculature, leaf hydathodes, anther filaments, stigma, sepal vasculature, at the base and apical parts of siliques, and replum. Expressed in the micropylar endosperm and radicle tip in ea
Database Links

KEGG: ath:AT5G66460

STRING: 3702.AT5G66460.1

UniGene: At.28865

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