mrc1 Antibody

Code CSB-PA889248XA01SXV
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Product Details

Full Product Name
Rabbit anti-Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) mrc1 Polyclonal antibody
Uniprot No.
Target Names
mrc1
Alternative Names
mrc1 antibody; SPAC694.06c antibody; Mediator of replication checkpoint protein 1 antibody; DNA replication checkpoint mediator mrc1 antibody
Raised in
Rabbit
Species Reactivity
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Immunogen
Recombinant Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) mrc1 protein
Immunogen Species
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Component of the replisome and is required for rad3-dependent activation of the checkpoint kinase cds1 in response to replication fork arrest. Phosphorylation allows it to mediate the activation of cds1.
Gene References into Functions
  1. Results identify S. pombe Mrc1 with major role in mediating efficient replication stalling. Its function is independent of the protein's checkpoint activity, but dependent on a helix-turn-helix DNA-binding domain. PMID: 26201080
  2. Results show a novel checkpoint-independent function of Mrc1 which may define early-firing origins among the potential origins scattered on the chromosome. PMID: 21518960
  3. The N-terminal region of the S. pombe replication checkpoint protein Mrc1 has a helix-loop-helix motif that is predicted to bind to DNA. [mrc1] PMID: 15471884
  4. S. pombe Tel2 plays important roles in the Mrc1-mediated replication checkpoint as well as in the Cds1-independent regulation of genome integrity. PMID: 17189249
  5. These results suggest that interactions of the Swi1-Swi3 complex and Hsk1 kinase with Mrc1 may play a role in cellular responses to stalled replication forks in fission yeast PMID: 19422421

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Subcellular Location
Nucleus. Note=Associated with chromatin.
Database Links
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