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Recombinant Schizosaccharomyces pombe Mediator of replication checkpoint protein 1 (mrc1), partial

Recombinant Schizosaccharomyces pombe Mediator of replication checkpoint protein 1 (mrc1), partial

Code	CSB-YP889248SXV
MSDS	MSDS
Size	Pls inquire
Source	Yeast
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Code	CSB-EP889248SXV
MSDS	MSDS
Size	Pls inquire
Source	E.coli
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Code	CSB-EP889248SXV-B
MSDS	MSDS
Size	Pls inquire
Source	E.coli
Conjugate	Avi-tag Biotinylated E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code	CSB-BP889248SXV
MSDS	MSDS
Size	Pls inquire
Source	Baculovirus
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Code	CSB-MP889248SXV
MSDS	MSDS
Size	Pls inquire
Source	Mammalian cell
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Product Details
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Customer Reviews and Q&A
Target Background

Product Details

Purity

>85% (SDS-PAGE)

Target Names

mrc1

Uniprot No.

Q9P7T4

Alternative Names

mrc1; SPAC694.06c; Mediator of replication checkpoint protein 1; DNA replication checkpoint mediator mrc1

Species

Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)

Protein Length

Partial

Tag Info

Tag type will be determined during the manufacturing process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer before Lyophilization

Tris/PBS-based buffer, 6% Trehalose, pH 8.0

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet

Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function

Component of the replisome and is required for rad3-dependent activation of the checkpoint kinase cds1 in response to replication fork arrest. Phosphorylation allows it to mediate the activation of cds1.

Gene References into Functions

Results identify S. pombe Mrc1 with major role in mediating efficient replication stalling. Its function is independent of the protein's checkpoint activity, but dependent on a helix-turn-helix DNA-binding domain. PMID: 26201080
Results show a novel checkpoint-independent function of Mrc1 which may define early-firing origins among the potential origins scattered on the chromosome. PMID: 21518960
The N-terminal region of the S. pombe replication checkpoint protein Mrc1 has a helix-loop-helix motif that is predicted to bind to DNA. [mrc1] PMID: 15471884
S. pombe Tel2 plays important roles in the Mrc1-mediated replication checkpoint as well as in the Cds1-independent regulation of genome integrity. PMID: 17189249
These results suggest that interactions of the Swi1-Swi3 complex and Hsk1 kinase with Mrc1 may play a role in cellular responses to stalled replication forks in fission yeast PMID: 19422421

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Subcellular Location

Nucleus. Note=Associated with chromatin.

Database Links

KEGG: spo:SPAC694.06c

STRING: 4896.SPAC694.06c.1

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