MYB75 Antibody

Code CSB-PA863725XA01DOA
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Product Details

Full Product Name
Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) MYB75 Polyclonal antibody
Uniprot No.
Target Names
MYB75
Alternative Names
MYB75 antibody; PAP1 antibody; SIAA1 antibody; At1g56650 antibody; F25P12.92 antibody; Transcription factor MYB75 antibody; Myb-related protein 75 antibody; AtMYB75 antibody; Production of anthocyanin pigment 1 protein antibody; Suc-induced anthocyanin accumulation locus 1 antibody
Raised in
Rabbit
Species Reactivity
Arabidopsis thaliana
Immunogen
Recombinant Arabidopsis thaliana MYB75 protein
Immunogen Species
Arabidopsis thaliana (Mouse-ear cress)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Transcription activator, when associated with BHLH12/MYC1, EGL3, or GL3. Promotes the synthesis of. phenylpropanoid-derived compounds such as anthocyanins and proanthocyanidin, probably together with GL3 and BHLH2. Regulates the expression of CHS, DFRA, LDOX, and BAN.
Gene References into Functions
  1. This work lays the foundation for dissecting the sucrose signaling pathway of PAP1 and contributes to understanding the interplay between sucrose signaling, anthocyanin biosynthesis, and stress responses PMID: 27248141
  2. Flowers of 35S:PAP1 transgenic plants produced the same or even higher levels of volatiles when exposed to a long-term high-temperature regime. PMID: 25402319
  3. Performance of a specialist aphid (Brevicoryne brassicae) was unaffected after feeding on oxMYB75 plants, whereas a specialist caterpillar (Pieris brassicae) gained significantly higher body mass when feeding on this plant. PMID: 24619996
  4. the production of anthocyanin pigment 1 (AtPAP1) transcription factor from Arabidopsis thaliana induces anthocyanin production in transgenic Taraxacum brevicorniculatum.[AtPAP1] PMID: 24556963
  5. Data indicate that miR828, TAS4-siRNA81(-), and its targets MYB transcription factors PAP1 and PAP2 are responsive to sucrose. PMID: 21533841
  6. MYB75 is nuclear localized and acts as a transcriptional activator. Loss of MYB75 function affects secondary cell wall structure and composition. PMID: 20807862
  7. In the same light intensity condition, the responses of anthocyanin levels and profiling to medium alternation were different between pap1-D and wild type plants. PMID: 20309578
  8. Two putative glycosyltransferase genes (At5g17050 and At4g14090) induced by PAP1 expression were confirmed to encode flavonoid 3-O-glucosyltransferase and anthocyanin 5-O-glucosyltransferase, respectively. PMID: 15807784
  9. High-temperature, low-light (HTLL) treatment of 35S:PAP1 Arabidopsis thaliana over-expressing the PAP1 (Production of Anthocyanin Pigment 1) gene results in reversible reduction of red colouration. PMID: 19192188

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Subcellular Location
Nucleus.
Tissue Specificity
Mostly expressed in mature plant aerial part. Detected ubiquitously, with higher levels in rosette leaves and flowers.
Database Links

KEGG: ath:AT1G56650

STRING: 3702.AT1G56650.1

UniGene: At.20447

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