Recombinant Arabidopsis thaliana Transcription factor MYB75 (MYB75)

Code CSB-YP863725DOA
MSDS
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Source Yeast
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Code CSB-EP863725DOA
MSDS
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Source E.coli
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Code CSB-EP863725DOA-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP863725DOA
MSDS
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Source Baculovirus
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Code CSB-MP863725DOA
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
MYB75
Uniprot No.
Alternative Names
MYB75; PAP1; SIAA1; At1g56650; F25P12.92; Transcription factor MYB75; Myb-related protein 75; AtMYB75; Production of anthocyanin pigment 1 protein; Suc-induced anthocyanin accumulation locus 1
Species
Arabidopsis thaliana (Mouse-ear cress)
Expression Region
1-248
Target Protein Sequence
MEGSSKGLRK GAWTTEEDSL LRQCINKYGE GKWHQVPVRA GLNRCRKSCR LRWLNYLKPS IKRGKLSSDE VDLLLRLHRL LGNRWSLIAG RLPGRTANDV KNYWNTHLSK KHEPCCKIKM KKRDITPIPT TPALKNNVYK PRPRSFTVNN DCNHLNAPPK VDVNPPCLGL NINNVCDNSI IYNKDKKKDQ LVNNLIDGDN MWLEKFLEES QEVDILVPEA TTTEKGDTLA FDVDQLWSLF DGETVKFD
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Transcription activator, when associated with BHLH12/MYC1, EGL3, or GL3. Promotes the synthesis of. phenylpropanoid-derived compounds such as anthocyanins and proanthocyanidin, probably together with GL3 and BHLH2. Regulates the expression of CHS, DFRA, LDOX, and BAN.
Gene References into Functions
  1. This work lays the foundation for dissecting the sucrose signaling pathway of PAP1 and contributes to understanding the interplay between sucrose signaling, anthocyanin biosynthesis, and stress responses PMID: 27248141
  2. Flowers of 35S:PAP1 transgenic plants produced the same or even higher levels of volatiles when exposed to a long-term high-temperature regime. PMID: 25402319
  3. Performance of a specialist aphid (Brevicoryne brassicae) was unaffected after feeding on oxMYB75 plants, whereas a specialist caterpillar (Pieris brassicae) gained significantly higher body mass when feeding on this plant. PMID: 24619996
  4. the production of anthocyanin pigment 1 (AtPAP1) transcription factor from Arabidopsis thaliana induces anthocyanin production in transgenic Taraxacum brevicorniculatum.[AtPAP1] PMID: 24556963
  5. Data indicate that miR828, TAS4-siRNA81(-), and its targets MYB transcription factors PAP1 and PAP2 are responsive to sucrose. PMID: 21533841
  6. MYB75 is nuclear localized and acts as a transcriptional activator. Loss of MYB75 function affects secondary cell wall structure and composition. PMID: 20807862
  7. In the same light intensity condition, the responses of anthocyanin levels and profiling to medium alternation were different between pap1-D and wild type plants. PMID: 20309578
  8. Two putative glycosyltransferase genes (At5g17050 and At4g14090) induced by PAP1 expression were confirmed to encode flavonoid 3-O-glucosyltransferase and anthocyanin 5-O-glucosyltransferase, respectively. PMID: 15807784
  9. High-temperature, low-light (HTLL) treatment of 35S:PAP1 Arabidopsis thaliana over-expressing the PAP1 (Production of Anthocyanin Pigment 1) gene results in reversible reduction of red colouration. PMID: 19192188

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Subcellular Location
Nucleus.
Tissue Specificity
Mostly expressed in mature plant aerial part. Detected ubiquitously, with higher levels in rosette leaves and flowers.
Database Links

KEGG: ath:AT1G56650

STRING: 3702.AT1G56650.1

UniGene: At.20447

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