CBP20 Antibody

Code CSB-PA893563XA01DOA
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Product Details

Full Product Name
Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) CBP20 Polyclonal antibody
Uniprot No.
Target Names
CBP20
Alternative Names
CBP20 antibody; At5g44200 antibody; MLN1.12 antibody; Nuclear cap-binding protein subunit 2 antibody; 20 kDa nuclear cap-binding protein antibody; NCBP 20 kDa subunit antibody; AtCBP20 antibody
Raised in
Rabbit
Species Reactivity
Arabidopsis thaliana
Immunogen
Recombinant Arabidopsis thaliana CBP20 protein
Immunogen Species
Arabidopsis thaliana (Mouse-ear cress)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Component of the cap-binding complex (CBC), which binds co-transcriptionally to the 5' cap of pre-mRNAs and is involved in various processes such as pre-mRNA splicing and RNA-mediated gene silencing (RNAi) by microRNAs (miRNAs). The CBC complex is involved in miRNA-mediated RNA interference and is required for primary miRNA processing. In the CBC complex, CBP20 recognizes and binds capped RNAs (m7GpppG-capped RNA) but requires ABH1/CBP80 to stabilize the movement of its N-terminal loop and lock the CBC into a high affinity cap-binding state with the cap structure. CBP20 also plays a role in stabilization of ABH1/CBP80 and ABH1/CBP80 localization to the nucleus. Involved in flowering regulation via its interaction with FRIGIDA.
Gene References into Functions
  1. Some hitherto undescribed changes were found in the tissue structure and epidermal morphology of cbp20 mutant PMID: 21143728
  2. The N-terminal part of AtCBP20 is crucial for the formation of a complex with AtCBP80. PMID: 19453442
Subcellular Location
Nucleus. Cytoplasm. Note=Predominantly nuclear.
Protein Families
RRM NCBP2 family
Tissue Specificity
Expressed in all tissues analyzed, including roots, stems, leaves and flowers.
Database Links

KEGG: ath:AT5G44200

STRING: 3702.AT5G44200.1

UniGene: At.23549

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