nkd1 Antibody

Code CSB-PA648794XA01DIL
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Product Details

Full Product Name
Rabbit anti-Danio rerio (Zebrafish) (Brachydanio rerio) nkd1 Polyclonal antibody
Uniprot No.
Target Names
nkd1
Alternative Names
nkd1 antibody; Protein naked cuticle homolog 1 antibody; Naked-1 antibody
Raised in
Rabbit
Species Reactivity
Danio rerio (Zebrafish) (Brachydanio rerio)
Immunogen
Recombinant Danio rerio (Zebrafish) (Brachydanio rerio) nkd1 protein
Immunogen Species
Danio rerio (Zebrafish) (Brachydanio rerio)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Cell autonomous antagonist of the canonical Wnt signaling pathway. May activate a second Wnt signaling pathway that controls planar cell polarity.
Gene References into Functions
  1. This work reveals that the zebrafish Nkd1 EF-hand is essential to balance Wnt signaling inputs and modulate the appropriate outputs, while the Drosophila-like EF-Hand primarily functions in beta-catenin signaling. PMID: 29180104
  2. Nkd1 activity is specifically dependent on Wnt ligand activation of the receptor. Nkd1 is recruited to the Wnt signalosome with Dvl2, where it becomes activated to move into the cytoplasm to interact with beta-catenin, inhibiting its nuclear accumulation. PMID: 25904337
  3. Nkd1 functions as a passive antagonist of Wnt signaling, functioning only when homeostatic levels of Wnt signaling have been breached or when Wnt signaling becomes destabilized. PMID: 24009776
  4. Data demonstrate that Nkd1 binds to beta-catenin and prevents its nuclear accumulation. Nkd1 function is dependent on its interaction with the cell membrane. PMID: 21490931
  5. Nkd1 knockdown in the dorsal forerunner cells causes beta-catenin nuclear localization/transcriptional activation & changes in DFC migration, Kupffer's vesicle formation, ciliogenesis, left-right patterning, & asymmetric charon expression. PMID: 20858476
  6. data indicate that zebrafish Nkd1 and Nkd2 function to limit both canonical and non-canonical Wnt signaling PMID: 17689523

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Subcellular Location
Cell membrane. Cytoplasm.
Protein Families
NKD family
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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Address
7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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