Recombinant Danio rerio Protein naked cuticle homolog 1 (nkd1)

Code CSB-YP648794DIL
MSDS
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Source Yeast
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Code CSB-EP648794DIL
MSDS
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Source E.coli
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Code CSB-EP648794DIL-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP648794DIL
MSDS
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Source Baculovirus
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Code CSB-MP648794DIL
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
nkd1
Uniprot No.
Alternative Names
nkd1; Protein naked cuticle homolog 1; Naked-1
Species
Danio rerio (Zebrafish) (Brachydanio rerio)
Expression Region
2-440
Target Protein Sequence
GKLHSKHAA ICKARESPEG DSFVVNACLA RKGLDDWMVK QKYYCSSSRV DQRDCQQKNN CELSPRDPLD EGYAEGISDE HYRLEVALPP EKTDSCSVEE RMQGTEGSAT VGPHKQLQFE ELECAVSVEE DNRQEWTFTL YDFDNNGKVT REDITSLLHT IYEVVDASVN HSPSSSKTLR VKLSVAPDSS QRWKNCTQTN TDTPNPKHKG EKCIEDSKTS EKKSRAFLRR YHAEHHSQQG CQRHCVDENL ERRNHYLDLA GIENYTSRFG TAAPATEPAK PTHATRSSNQ SRSRSQEPEN GQVHPAHHRR SQTMSTDPPA ILTRHTHALR SPKTHRTPPT QASAGRVMRR GAPPPPSVPN QTPPHQSSGP YRRHKQRPKE ALHYRALGPS VTSGPVLEKE HVRDLPSLVL YEGGLAQVVQ RHEHHHHHEH HHHYHHFYQS
Protein Length
Full Length of Mature Protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Cell autonomous antagonist of the canonical Wnt signaling pathway. May activate a second Wnt signaling pathway that controls planar cell polarity.
Gene References into Functions
  1. This work reveals that the zebrafish Nkd1 EF-hand is essential to balance Wnt signaling inputs and modulate the appropriate outputs, while the Drosophila-like EF-Hand primarily functions in beta-catenin signaling. PMID: 29180104
  2. Nkd1 activity is specifically dependent on Wnt ligand activation of the receptor. Nkd1 is recruited to the Wnt signalosome with Dvl2, where it becomes activated to move into the cytoplasm to interact with beta-catenin, inhibiting its nuclear accumulation. PMID: 25904337
  3. Nkd1 functions as a passive antagonist of Wnt signaling, functioning only when homeostatic levels of Wnt signaling have been breached or when Wnt signaling becomes destabilized. PMID: 24009776
  4. Data demonstrate that Nkd1 binds to beta-catenin and prevents its nuclear accumulation. Nkd1 function is dependent on its interaction with the cell membrane. PMID: 21490931
  5. Nkd1 knockdown in the dorsal forerunner cells causes beta-catenin nuclear localization/transcriptional activation & changes in DFC migration, Kupffer's vesicle formation, ciliogenesis, left-right patterning, & asymmetric charon expression. PMID: 20858476
  6. data indicate that zebrafish Nkd1 and Nkd2 function to limit both canonical and non-canonical Wnt signaling PMID: 17689523

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Subcellular Location
Cell membrane. Cytoplasm.
Protein Families
NKD family
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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