Notum Antibody

Code CSB-PA784750XA01DLU
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Product Details

Full Product Name
Rabbit anti-Drosophila melanogaster (Fruit fly) Notum Polyclonal antibody
Uniprot No.
Target Names
Notum
Alternative Names
Notum antibody; wf antibody; CG13076 antibody; Palmitoleoyl-protein carboxylesterase NOTUM antibody; EC 3.1.1.98 antibody; Protein Notum antibody; Protein wingful antibody; dNOTUM antibody
Raised in
Rabbit
Species Reactivity
Drosophila melanogaster (Fruit fly)
Immunogen
Recombinant Drosophila melanogaster (Fruit fly) Notum protein
Immunogen Species
Drosophila melanogaster (Fruit fly)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Carboxylesterase that acts as a key negative regulator of the Wnt signaling pathway by specifically mediating depalmitoleoylation of WNT proteins. Serine palmitoleoylation of WNT proteins is required for efficient binding to frizzled receptors. Also acts as a regulator of long-range activity of Hedgehog (hh), possibly by regulating the switch between low and high level hh pathway signaling.
Gene References into Functions
  1. Notum requires glypicans to suppress Wnt signalling, but does not cleave their glycophosphatidylinositol anchor; structural analyses reveal glycosaminoglycan binding sites on Notum, which probably help Notum to co-localize with Wnt proteins PMID: 25731175
  2. Hydrolysis of the Dally-GPI by Notum provides a switch from low to high level signalling by promoting internalisation of the Hh-Patched ligand-receptor complex. PMID: 22872085
  3. notum can cleave mammalian glypticans and GPI-anchored proteins from the cell surface. PMID: 17967162
  4. Wingful (wf) encodes a potent extracellular feedback inhibitor of Wg. In contrast to the cytoplasmic protein Naked cuticle (Nkd), the only known Wg feedback antagonist, Wf functions during larval stages. PMID: 12000788
  5. Identification of a secreted repressor of Wingless activity, which we call Notum. PMID: 12015973

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Subcellular Location
Secreted. Cell surface.
Protein Families
Pectinacetylesterase family, Notum subfamily
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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Address
7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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