NRPB2 Antibody

Code CSB-PA018328XA01DOA
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Product Details

Full Product Name
Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) NRPB2 Polyclonal antibody
Uniprot No.
Target Names
NRPB2
Alternative Names
NRPB2 antibody; EMB1989 antibody; RP140 antibody; RPB135 antibody; RPB2 antibody; At4g21710 antibody; F17L22.170 antibody; DNA-directed RNA polymerase II subunit 2 antibody; DNA-directed RNA polymerase II 135 kDa polypeptide antibody; DNA-directed RNA polymerase II subunit RPB2 antibody; RNA polymerase II subunit 2 antibody; RNA polymerase II subunit B2 antibody; EC 2.7.7.6 antibody; Protein EMBRYO DEFECTIVE 1989 antibody
Raised in
Rabbit
Species Reactivity
Arabidopsis thaliana
Immunogen
Recombinant Arabidopsis thaliana NRPB2 protein
Immunogen Species
Arabidopsis thaliana (Mouse-ear cress)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Second largest component of RNA polymerase II which synthesizes mRNA precursors and many functional non-coding RNAs. Proposed to contribute to the polymerase catalytic activity and forms the polymerase active center together with the largest subunit. Pol II is the central component of the basal RNA polymerase II transcription machinery. It is composed of mobile elements that move relative to each other. NRPB2 is part of the core element with the central large cleft, the clamp element that moves to open and close the cleft and the jaws that are thought to grab the incoming DNA template.; Essential for the completion of the three rounds of mitosis in female megaspores required for the development of mature gametophytes.
Subcellular Location
Nucleus.
Protein Families
RNA polymerase beta chain family
Database Links

KEGG: ath:AT4G21710

STRING: 3702.AT4G21710.1

UniGene: At.112

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