Nf2 Antibody

Code CSB-PA015741ZA01MO
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Product Details

Full Product Name Rabbit anti-Mus musculus (Mouse) Nf2 Polyclonal antibody
Uniprot No. P46662
Target Names Nf2
Alternative Names Merlin (Moesin-ezrin-radixin-like protein) (Neurofibromin-2) (Schwannomin), Nf2, Nf-2
Raised in Rabbit
Species Reactivity Mus musculus
Immunogen Recombinant Mus musculus Nf2 protein
Immunogen Species Mus musculus (Mouse)
Conjugate Non-conjugate
Clonality Polyclonal
Isotype IgG
Purification Method Protein A/G
Concentration It differs from different batches. Please contact us to confirm it.
Buffer Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form Liquid
Tested Applications ELISA, WB (ensure identification of antigen)
Protocols ELISA Protocol
Troubleshooting and FAQs Antibody FAQs
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables ① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee ① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time Made-to-order (12-14 weeks)

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Target Background

Function
(From Uniprot)
Probable regulator of the Hippo/SWH (Sav/Wts/Hpo) signaling pathway, a signaling pathway that plays a pivotal role in tumor suppression by restricting proliferation and promoting apoptosis. Along with WWC1 can synergistically induce the phosphorylation of LATS1 and LATS2 and can probably function in the regulation of the Hippo/SWH (Sav/Wts/Hpo) signaling pathway. May act as a membrane stabilizing protein. May inhibit PI3 kinase by binding to AGAP2 and impairing its stimulating activity. Suppresses cell proliferation and tumorigenesis by inhibiting the CUL4A-RBX1-DDB1-VprBP/DCAF1 E3 ubiquitin-protein ligase complex. Plays a role in lens development and is required for complete fiber cell terminal differentiation, maintenance of cell polarity and separation of the lens vesicle from the corneal epithelium.
Gene References into Functions
  1. Results demonstrate that loss of merlin function in Schwann Cells (SCs) leads to increased endoneurial space and ultrastructural myelin alterations in the sciatic nerve before and after injury. Loss of merlin function in SCs delays neural recovery following injury. Nevertheless, there were no significant functional or electrophysiological differences in neural regeneration attributable to Nf2 mutation in SCs. PMID: 29408605
  2. Data indicate an essential role for NF2 and the Hippo pathway in regulation of branching morphogenesis in the mammalian kidney. PMID: 27480037
  3. Loss of Nf2 causes hyperplasia and ocular abnormalities. PMID: 29249622
  4. studies suggest that NF2 normally limits biliary morphogenesis by coordinating lumen expansion and cell architecture. This work provides fundamental insight into how biliary fate and tubulogenesis are coordinated during development and will guide analyses of disease-associated and experimentally induced biliary pathologies. PMID: 29712669
  5. Data show that early Smarcb1 loss causes rhabdoid tumors whereas loss at later stages combined with Nf2 gene inactivation causes shwannomas. PMID: 28824165
  6. Rho attenuates the interaction between Amot and Nf2 by binding to the coiled-coil domain of Amot. PMID: 28947533
  7. co-deletion of Rac1 with Nf2 blocks tumor initiation but paradoxically exacerbates hepatomegaly induced by Nf2 loss, which can be suppressed either by treatment with pro-oxidants or by co-deletion of Yap. PMID: 27818180
  8. Merlin controls the repair capacity of Schwann cells after injury by regulating Hippo/YAP signaling activity. PMID: 28137778
  9. NF2 is activated by oxidative stress in cardiomyocytes and myocardium and facilitates apoptosis. PMID: 27402866
  10. loss of axonal contact following nerve injury results in merlin phosphorylation leading to increased p75(NTR) expression. PMID: 26057084
  11. Loss of Nf2 and Cdkn2a/b have synergistic effects with PDGF-B overexpression promoting meningioma malignant transformation. PMID: 26418719
  12. Merlin 1 and 2 act as tumor suppressors and are required for optimal sperm maturation PMID: 26258444
  13. Together our results uncover miRNAs as yet another negative mechanism controlling Merlin tumor suppressor functions. PMID: 26549232
  14. Merlin and Ezrin are components of a mechanism where mechanical forces associated with cell junctions are transduced across the cell cortex via cortical actomyosin cytoskeleton to control lateral mobility and activity of epidermal growth factor receptor. PMID: 26483553
  15. The study describe a novel NF2 mouse model recapitulating schwannoma phenotypes found in human patients where tumors develop in the cranial nerve VIII and/or the spinal roots. PMID: 25113746
  16. Nf2/Merlin controls spinal cord neural progenitor function in a Rac1/ErbB2-dependent manner. PMID: 24817309
  17. Nf2-Yap signaling plays important roles in controlling the expansion of dorsal root ganglia progenitors and glia during DRG development PMID: 25433207
  18. CD44 cytoplasmic tail cleaved by RIP could release DCAF1 from merlin by competing for binding to the merlin FERM domain, which results in the inhibition of merlin-mediated suppression of tumorigenesis. PMID: 24912773
  19. Findings indicate that merlin is sumoylated and that this post-translational modification is essential for tumor suppression. PMID: 24166499
  20. Cdc42 regulates SC radial sorting in vivo through Neurofibromin 2/merlin dependent signaling pathways PMID: 24014231
  21. The data establish a clear role for Nf2 upstream of Yap in the preimplantation embryo and demonstrate that Hippo signaling is essential to segregate the inner cell mass from the trophectoderm. PMID: 23791728
  22. These results indicate that in both Drosophila and mammals, Merlin activates Wts/Lats phosphorylation without stimulating the intrinsic kinase activity of Hpo/Mst. PMID: 24012335
  23. Meningioma progression in mice triggered by Nf2 and Cdkn2ab inactivation. PMID: 23045274
  24. Signaling between Nf2 and Rac1 occurs in a bidirectional fashion, and these interactions are modulated by PKA. PMID: 23045281
  25. Nf2 is required for hippocampal morphogenesis. Yap/Taz are key downstream effectors of Nf2 during brain development. PMID: 23863479
  26. Neurofibromin 2 is an AKAP (A-kinase-anchoring protein) scaffold protein that facilitates the function of the cAMP/PKA-LATS-YAP pathway. PMID: 23644383
  27. propose that Merlin mediates contact inhibition and suppresses tumorigenesis by translocating to the nucleus to inhibit CRL4(DCAF1). PMID: 21878678
  28. Results demonstrate that Schwannomin plays an essential role in inducing and/or maintaining the SC's spindle shape, and by stabilizing the bipolar morphology, Sch promotes the alignment of SCs with axons and ultimately influences myelin segment length. PMID: 21182951
  29. FERM domain-mediated phosphoinositide binding and membrane association are critical for the growth-regulatory function of merlin PMID: 21402777
  30. Depletion of Angiomotin in Nf2(-/-) Schwann cells attenuates the Ras-MAPK signaling pathway, impedes cellular proliferation in vitro and tumorigenesis in vivo PMID: 21481793
  31. Neurofibromatosis type 2 protein is inactivated in glioblastoma cells by overexpression of ezrin. PMID: 20156804
  32. Results reveal that Merlin can associate directly with alpha-catenin and link it to Par3, thereby providing an essential link between the AJ and the Par3 polarity complex during junctional maturation. PMID: 21074722
  33. a critical role for Nf2/Merlin in controlling homeostasis of the liver stem cell niche PMID: 20675406
  34. Nf2-deficient phenotypes in multiple tissues were largely suppressed by heterozygous deletion of Yap, suggesting that YAP is a major effector of Merlin/NF2 in growth regulation. PMID: 20643348
  35. This study provided merlin plays a pivotal role in controlling the neuronal wiring in the developing CNS. PMID: 20668201
  36. Tumor suppression by merlin is independent of its role as an organizer of the actin cytoskeleton in Schwann cells. PMID: 19910496
  37. Rac1-mediated canonical Wnt signaling is essential for the loss of contact inhibition in NF2-deficient cells. PMID: 20154721
  38. Structural basis for neurofibromatosis type 2 PMID: 11756419
  39. phosphorylation of merlin at serine 518 leads to dramatic protein relocalization PMID: 11782491
  40. Nf2 and p53 mutations do not synergize in meningeal tumorigenesis.Nf2 gene inactivation in arachnoidal cells is rate-limiting for meningioma development in the mouse. PMID: 12000789
  41. Mutant products of the NF2 tumor suppressor gene are degraded by the ubiquitin-proteasome pathway PMID: 12130630
  42. A FERM domain mutant of the Nf2 tumor suppressor gene causes cellular transformation and tumorigenesis in mice PMID: 12203111
  43. Merlin inhibits Pak1;loss of Merlin leads to inappropriate activation of Pak1. Conversely, the overexpression of Merlin leads to inhibition of Pak1 activation. PMID: 14580336
  44. biallelic loss of Nf2 function in neural crest-derived cells hemizygous for p53 results in resistance to osteogenic tumours and increased susceptibility to peripheral nerve sheath tumours PMID: 15221010
  45. erbin regulates MAP kinase activation in Schwann cells and suggest that erbin links merlin to both adherens junction protein complexes and the MAP kinase signaling pathway. PMID: 15659388
  46. NF2 tumor suppressor Merlin and the ERM proteins interact with N-WASP and regulate its actin polymerization function PMID: 15699051
  47. In brain tissue and neuronal progenitor cell cultures merlin was predominantly found in neurons. Merlin expression was seen from Day 11 in mouse embryos. PMID: 15797715
  48. These results suggest that merlin contributes, via its protein-to-protein interaction with Grb2 and consequent inhibition of the MAPK pathways. PMID: 16405865
  49. PP1-delta and MYPT-1 were co-precipitated with endogenous merlin from NIH-3T3 cells PMID: 16885985
  50. inhibition of the CD44-HA interaction contributes to the tumor-suppressor function of merlin, and they suggest that merlin inhibits tumor growth, at least in part, by negatively regulating CD44 function PMID: 16953231

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Subcellular Location Cell membrane; Peripheral membrane protein; Cytoplasmic side. Cell projection. Cytoplasm, cytoskeleton. Nucleus.
Database Links

KEGG: mmu:18016

STRING: 10090.ENSMUSP00000105536

UniGene: Mm.297109

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