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ofd1 Antibody

Datasheet
Code CSB-PA612106XA01SXV
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Product Details

Full Product Name
Rabbit anti-Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) ofd1 Polyclonal antibody
Uniprot No.
Q11120
Target Names
ofd1
Alternative Names
ofd1 antibody; SPBC6B1.08c antibody; Prolyl 3,4-dihydroxylase ofd1 antibody; EC 1.14.11.- antibody; 2-oxoglutarate and Fe(II) dioxygenase domain-containing protein 1 antibody; PKHD-type hydroxylase ofd1 antibody; uS12 prolyl 3,4-dihydroxylase antibody
Raised in
Rabbit
Species Reactivity
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Immunogen
Recombinant Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) ofd1 protein
Immunogen Species
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
ELISA Protocol
Troubleshooting and FAQs
Antibody FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)
Usage
For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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ofd1 Proteins

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Target Background

Function
Prolyl 3,4-dihydroxylase that catalyzes 3,4-dihydroxylation of 'Pro-62' of small ribosomal subunit uS12 (rps23 and rps2302), thereby regulating protein translation termination efficiency. Negative regulator of the stability of the N-terminal transcription factor domain (Sre1N) of sre1 which is released from the membrane and enters the nucleus to activate hypoxic gene expression.
Gene References into Functions
  1. hydroxylase Ofd1 regulates the Sre1 hypoxic transcription factor in fission yeast by controlling DNA binding.[Sre1; ofd1] PMID: 22017871
  2. The Ofd1 N-terminal dioxygenase domain is required for oxygen sensing and the Ofd1 C-terminal domain accelerates Sre1N degradation.(Sre1)(Ofd1) PMID: 18418381
Subcellular Location
Cytoplasm. Nucleus.
Protein Families
TPA1 family
Database Links
Promotions
Reward For Reviews
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