Recombinant Schizosaccharomyces pombe PKHD-type hydroxylase ofd1 (ofd1), partial

Code CSB-YP612106SXV
MSDS
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Source Yeast
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Code CSB-EP612106SXV
MSDS
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Source E.coli
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Code CSB-EP612106SXV-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP612106SXV
MSDS
Size Pls inquire
Source Baculovirus
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Code CSB-MP612106SXV
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
ofd1
Uniprot No.
Alternative Names
ofd1; SPBC6B1.08c; Prolyl 3,4-dihydroxylase ofd1; EC 1.14.11.-; 2-oxoglutarate and Fe(II) dioxygenase domain-containing protein 1; PKHD-type hydroxylase ofd1; uS12 prolyl 3,4-dihydroxylase
Species
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Prolyl 3,4-dihydroxylase that catalyzes 3,4-dihydroxylation of 'Pro-62' of small ribosomal subunit uS12 (rps23 and rps2302), thereby regulating protein translation termination efficiency. Negative regulator of the stability of the N-terminal transcription factor domain (Sre1N) of sre1 which is released from the membrane and enters the nucleus to activate hypoxic gene expression.
Gene References into Functions
  1. hydroxylase Ofd1 regulates the Sre1 hypoxic transcription factor in fission yeast by controlling DNA binding.[Sre1; ofd1] PMID: 22017871
  2. The Ofd1 N-terminal dioxygenase domain is required for oxygen sensing and the Ofd1 C-terminal domain accelerates Sre1N degradation.(Sre1)(Ofd1) PMID: 18418381
Subcellular Location
Cytoplasm. Nucleus.
Protein Families
TPA1 family
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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Address
7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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