par-3 Antibody

Code CSB-PA622011XA01CXY
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Product Details

Full Product Name
Rabbit anti-Caenorhabditis elegans par-3 Polyclonal antibody
Uniprot No.
Target Names
par-3
Alternative Names
par-3 antibody; F54E7.3Partitioning defective protein 3 antibody; Abnormal embryonic partitioning of cytoplasm protein 3 antibody
Raised in
Rabbit
Species Reactivity
Caenorhabditis elegans
Immunogen
Recombinant Caenorhabditis elegans par-3 protein
Immunogen Species
Caenorhabditis elegans
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
In cooperation with pkc-3, required for establishing cell polarity and regulating spindle orientation in the early embryo. Localization is crucial for recruiting par-6 and pkc-3 to the peripheral apical cortex and restricting par-2 to basolateral surfaces. Necessary for apicobasal and anterior-posterior asymmetries associated with cell adhesion and gastrulation during the first few cycles of embryogenesis, and also for epithelial cell polarity in the distal spermatheca. Regulates the asymmetric localization of csnk-1, ppk-1 and gpr-1/2 during the first embryonic division.
Gene References into Functions
  1. a model of interactions between the Par proteins that includes both mutual inhibition and PAR-3 oligomerization PMID: 21943422
  2. Different domains of C. elegans par-3 are required at different times in development. PMID: 20678977
  3. PAR-3 foci move apically and cluster, and PAR-3 is required to assemble E-cadherin into foci and for foci to accumulate at the apical surface. PMID: 20431121
  4. PAR-3 activity is required for the proper polarization of spermathecal cells and that defective ovulation results from defective distal spermathecal development. PMID: 15151982
  5. PAR-3 and PAR-1 have graded cortical distributions with the highest levels at the anterior and posterior poles, respectively, and the lowest levels of these proteins correlate with high LET-99 accumulation PMID: 17761536

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Subcellular Location
Cytoplasm. Note=Cytoplasmic and cell periphery.
Protein Families
PAR3 family
Tissue Specificity
Asymmetrically distributed at the periphery of the zygote and in dividing blastomeres of the germline lineage. Coexpressed with par-6; patchy expression observed at the periphery after completion of meiosis I and in meiosis II. On completion of metaphase
Database Links

STRING: 6239.F54E7.3b.2

UniGene: Cel.5328

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