PCNA Antibody

Code CSB-PA017621XA01DLU
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Product Details

Full Product Name
Rabbit anti-Drosophila melanogaster (Fruit fly) PCNA Polyclonal antibody
Uniprot No.
Target Names
PCNA
Alternative Names
PCNA antibody; mus209 antibody; PCNA1 antibody; CG9193 antibody; Proliferating cell nuclear antigen antibody; PCNA antibody; Cyclin antibody; Mutagen-sensitive 209 protein antibody
Raised in
Rabbit
Species Reactivity
Drosophila melanogaster (Fruit fly)
Immunogen
Recombinant Drosophila melanogaster (Fruit fly) PCNA protein
Immunogen Species
Drosophila melanogaster (Fruit fly)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Likely to be an auxiliary protein of DNA polymerase delta complex and is probably involved in the control of DNA replication and repair by increasing the polymerase's processibility.
Gene References into Functions
  1. crystal of DmPCNA diffracted to 2.0 A resolution and belonged to space group H3, with unit-cell parameters a = b = 151.16, c = 38.28 A PMID: 23545643
  2. We observed that SUUR chromosomal localization changed along with PCNA pattern and these proteins largely co-localized during the late S-phase in salivary glands. PMID: 23149855
  3. E2F regulation of PCNA is dispensable for viability, but is nonetheless important for normal Drosophila development. PMID: 12526745
  4. A Q123V mutation dramaticly enhances the abilities of Drosophila PCNA to stimulate calf thymus pol delta. Replacing the entire interdomain connector loop AA 119-133 with the corresponding residues from human PCNA results in additional enhancement. PMID: 15310391
Subcellular Location
Nucleus. Chromosome. Cytoplasm.
Protein Families
PCNA family
Tissue Specificity
Expressed at high levels in adult ovary.
Database Links

KEGG: dme:Dmel_CG9193

STRING: 7227.FBpp0085619

UniGene: Dm.4751

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