PDAT1 Antibody

Datasheet

Code	CSB-PA887822XA01DOA
Size	US$299
Image	Western Blot Positive WB detected in Recombinant protein All lanes: PDAT1 antibody at 1:2000 Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 26.5 kDa Observed band size: 35 kDa
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Product Details

Full Product Name

Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) PDAT1 Polyclonal antibody

Uniprot No.

Q9FNA9

Target Names

PDAT1

Alternative Names

PDAT1 antibody; PDAT antibody; At5g13640 antibody; MSH12.10 antibody; T6I14.2 antibody; Phospholipid:diacylglycerol acyltransferase 1 antibody; AtPDAT antibody; EC 2.3.1.158 antibody

Raised in

Rabbit

Species Reactivity

Arabidopsis thaliana

Immunogen

Recombinant Arabidopsis thaliana PDAT1 protein

Immunogen Species

Arabidopsis thaliana (Mouse-ear cress)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Protein A/G

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB

Protocols

ELISA Protocol
Western Blotting(WB) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

The PDAT1 polyclonal antibody can be raised by immunizing a rabbit with the recombinant Arabidopsis thaliana PDAT1 protein. The PDAT1 antibody is collected from the rabbit serum and then purified through protein A/G. This PDAT1 antibody is suitable for the recognition of Arabidopsis thaliana PDAT1 protein in the ELISA and WB applications.

The main function of Arabidopsis thaliana PDAT1 protein is to catalyze the transfer of a fatty acyl group from a phospholipid to a diacylglycerol molecule, resulting in the synthesis of triacylglycerol (TAG). This process is known as phospholipid: diacylglycerol acyltransferase (PDAT) activity. PDAT1 is specifically involved in the synthesis of TAG in the chloroplasts of Arabidopsis thaliana, which serves as an important energy storage molecule in plants.

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Target Background

Function

Triacylglycerol formation by an acyl-CoA independent pathway. The enzyme preferentially transfers acyl groups from the sn-2 position of a phospholipid to diacylglycerol, thus forming an sn-1-lysophospholipid. Involved in epoxy and hydroxy fatty acid accumulation in seeds. Has complementary functions with DAG1 that are essential for triacylglycerol synthesis and normal development of both seeds and pollen.

Gene References into Functions

PDAT1 expression is regulated by MYB96 in the Arabidopsis seeds. PMID: 29660088
PDAT, transferring a fatty acyl moiety from phosphatidylcholine (PC) to diacylglycerol (DAG), was shown to be essential for heat-induced TAG accumulation. Finally, we show that pdat1mutant lines unable to accumulate TAGs under heat-stress conditions display normal short-term acquired thermotolerance but are compromised in basal thermotolerance. PMID: 28733391
PDAT1 plays a critical role in mediating triacylglycerol (TAG) synthesis and thereby protecting against free fatty acids (FFAs)-induced cell death in fast-growing tissues of plants. PMID: 24118513
PDAT1 and DGAT1 have overlapping functions for triacylglycerol synthesis in both seed and pollen, and that the absence of their function leads not only to a reduction in TAG, but also to critical defects in normal pollen and embryo development. [PDAT1] PMID: 20040537

Subcellular Location

Membrane; Single-pass membrane protein.

Protein Families

AB hydrolase superfamily, Lipase family

Tissue Specificity

Ubiquitous. Highest expression in young developing seeds.

Database Links

KEGG: ath:AT5G13640

STRING: 3702.AT5G13640.1

UniGene: At.22329

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

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Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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PDAT1 Antibody

Product Details

Related Products

Customer Reviews and Q&A

Target Background