PLB1 Antibody

Code CSB-PA334517XA01SVG
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Product Details

Full Product Name
Rabbit anti-Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) PLB1 Polyclonal antibody
Uniprot No.
Target Names
PLB1
Alternative Names
PLB1 antibody; YMR008C antibody; YM8270.10C antibody; Lysophospholipase 1 antibody; EC 3.1.1.5 antibody; Phospholipase B 1 antibody
Raised in
Rabbit
Species Reactivity
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Immunogen
Recombinant Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) PLB1 protein
Immunogen Species
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Sequentially removes both fatty acyl groups from diacylglycerophospholipids and therefore has both phospholipase B and lysophospholipase activities. It also displays transacylase activity. Substrate preference is phosphatidylserine > phosphatidylinositol >> phosphatidylcholine > phosphatidylethanolamine. The substrate specificity is pH- and ion-dependent. In contrast with activities observed at optimum pH 3.5, the order of substrate preference at pH 5.5 is phosphatidylcholine = phosphatidylethanolamine >> phosphatidylinositol. Degrades predominantly phosphatidylcholine and to some extent phosphatidylinositol in vivo.
Subcellular Location
Cell membrane; Lipid-anchor, GPI-anchor.
Protein Families
Lysophospholipase family
Database Links

KEGG: sce:YMR008C

STRING: 4932.YMR008C

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