Product Details

Full Product Name

Rabbit anti-Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) PPN1 Polyclonal antibody

Uniprot No.

Q04119

Target Names

PPN1

Alternative Names

PPN1 antibody; PHM5 antibody; YDR452W antibody; D9461.37 antibody; Endopolyphosphatase antibody; EC 3.6.1.10 antibody; Deoxyadenosine triphosphate phosphohydrolase antibody; dATP phosphohydrolase antibody; EC 3.6.1.- antibody; Exopolyphosphatase antibody; EC 3.6.1.11 antibody; Phosphate metabolism protein 5 antibody

Raised in

Rabbit

Species Reactivity

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Immunogen

Recombinant Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) PPN1 protein

Immunogen Species

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Target Background

Function

Catalyzes the hydrolysis of inorganic polyphosphate (polyP) chains of many hundreds of phosphate residues into shorter lengths both by cleaving phosphate from the chain end and by fragmenting long-chain polymers into shorter ones. The limited digestion products are 1 and 3 P(i) residues. Also releases phosphate from dATP. dATP phosphohydrolase activity is about 7-fold lower than the exopolyphosphatase activity (Ref.16).

Gene References into Functions

endopolyphosphatase cleaved inorganic polyphosphate without the release of orthophosphate and was inhibited by heparin PMID: 21314609
Both termini of prePpn1 and the post-translational modification of N-glycosylations are essential for the protease-mediated maturation of Ppn1. PMID: 15792812
Inactivation of the PPX1 gene encoding exopolyphosphatase had no effect on respiration functions and on polyphosphate level and chain length in mitochondria. PMID: 15925310
In the single PPN1 mutant, a considerable decrease of exopolyphosphatase activity was observed in all the compartments under study. PMID: 16779667
It has been shown that the high molecular mass exopolyphosphatase localized in cytosol of the yeast Saccharomyces cerevisiae is encoded by the PPN1 gene. PMID: 17009950
Influence of carbon source on the activity of PPN1. PMID: 18294131
S. cerevisiae possesses a system, which makes it possible to compensate for inactivation of the PPX1 and PPN1 genes encoding exopolyphosphatases of the yeast cells. PMID: 18976214
Exopolyphosphatase incoded by ppx1 and ppn1 genes do not take part in polyphsphate biosynthesis. PMID: 19580157
PPX1 and PPN1 genes encoding exopolyphosphatases. PMID: 19817683

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Subcellular Location

Vacuole membrane; Single-pass type II membrane protein. Cytoplasm.

Protein Families

Endopolyphosphatase PPN1 family

Database Links

KEGG: sce:YDR452W

STRING: 4932.YDR452W

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PPN1 Antibody

Product Details

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Target Background