RAD50 Antibody

Code CSB-PA874457XA01DOA
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Product Details

Full Product Name
Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) RAD50 Polyclonal antibody
Uniprot No.
Target Names
RAD50
Alternative Names
RAD50 antibody; At2g31970 antibody; F22D22.28 antibody; DNA repair protein RAD50 antibody; AtRAD50 antibody; EC 3.6.-.- antibody
Raised in
Rabbit
Species Reactivity
Arabidopsis thaliana
Immunogen
Recombinant Arabidopsis thaliana RAD50 protein
Immunogen Species
Arabidopsis thaliana (Mouse-ear cress)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Implicated in double-strand breaks (DSBs) repair by non-homologous end joining (NHEJ). Involved in telomere maintenance. Involved in telomerase action on chromosome ends. Required during meiosis for both male and female gametophytic development, for pairing and synapsis of homologous chromosomes during the early stages of meiotic recombination, especially during the pachytene stage of the first division.
Gene References into Functions
  1. Data show that the product of the PHS1 gene is a cytoplasmic protein that functions by controlling transport of RAD50 from cytoplasm to the nucleus. PMID: 19918061
  2. The protective role of Rad50 protein on shortened telomeres results from its action in constraining recombination to sister chromatids and thus avoiding end-to-end interactions. PMID: 16990794
Subcellular Location
Nucleus. Chromosome, telomere.
Protein Families
SMC family, RAD50 subfamily
Tissue Specificity
Widely expressed, predominantly in meristematic and reproductive tissues.
Database Links

KEGG: ath:AT2G31970

STRING: 3702.AT2G31970.1

UniGene: At.65745

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