Recombinant Arabidopsis thaliana DNA repair protein RAD50 (RAD50), partial

Code CSB-YP874457DOA
MSDS
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Source Yeast
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Code CSB-EP874457DOA
MSDS
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Source E.coli
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Code CSB-EP874457DOA-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP874457DOA
MSDS
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Source Baculovirus
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Code CSB-MP874457DOA
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
RAD50
Uniprot No.
Alternative Names
RAD50; At2g31970; F22D22.28; DNA repair protein RAD50; AtRAD50; EC 3.6.-.-
Species
Arabidopsis thaliana (Mouse-ear cress)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Implicated in double-strand breaks (DSBs) repair by non-homologous end joining (NHEJ). Involved in telomere maintenance. Involved in telomerase action on chromosome ends. Required during meiosis for both male and female gametophytic development, for pairing and synapsis of homologous chromosomes during the early stages of meiotic recombination, especially during the pachytene stage of the first division.
Gene References into Functions
  1. Data show that the product of the PHS1 gene is a cytoplasmic protein that functions by controlling transport of RAD50 from cytoplasm to the nucleus. PMID: 19918061
  2. The protective role of Rad50 protein on shortened telomeres results from its action in constraining recombination to sister chromatids and thus avoiding end-to-end interactions. PMID: 16990794
Subcellular Location
Nucleus. Chromosome, telomere.
Protein Families
SMC family, RAD50 subfamily
Tissue Specificity
Widely expressed, predominantly in meristematic and reproductive tissues.
Database Links

KEGG: ath:AT2G31970

STRING: 3702.AT2G31970.1

UniGene: At.65745

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