Rad9a Antibody

Code CSB-PA896673XA01MO
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Product Details

Full Product Name
Rabbit anti-Mus musculus (Mouse) Rad9a Polyclonal antibody
Uniprot No.
Target Names
Rad9a
Alternative Names
Rad9a antibody; Rad9 antibody; Cell cycle checkpoint control protein RAD9A antibody; mRAD9 antibody; EC 3.1.11.2 antibody; DNA repair exonuclease rad9 homolog A antibody; Rad9-like protein antibody
Raised in
Rabbit
Species Reactivity
Mus musculus (Mouse)
Immunogen
Recombinant Mus musculus (Mouse) Rad9a protein
Immunogen Species
Mus musculus (Mouse)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Component of the 9-1-1 cell-cycle checkpoint response complex that plays a major role in DNA repair. The 9-1-1 complex is recruited to DNA lesion upon damage by the RAD17-replication factor C (RFC) clamp loader complex. Acts then as a sliding clamp platform on DNA for several proteins involved in long-patch base excision repair (LP-BER). The 9-1-1 complex stimulates DNA polymerase beta (POLB) activity by increasing its affinity for the 3'-OH end of the primer-template and stabilizes POLB to those sites where LP-BER proceeds; endonuclease FEN1 cleavage activity on substrates with double, nick, or gap flaps of distinct sequences and lengths; and DNA ligase I (LIG1) on long-patch base excision repair substrates. The 9-1-1 complex is necessary for the recruitment of RHNO1 to sites of double-stranded breaks (DSB) occurring during the S phase. RAD9A possesses 3'->5' double stranded DNA exonuclease activity.
Gene References into Functions
  1. Rad9a is indispensable for spermatogonia differentiation and testicular development in mice. PMID: 27861152
  2. RAD9 has a prominent role in the ATR-Chk1 pathway that is necessary for successful formation of the damage-sensing complex and DNA damage checkpoint signaling. PMID: 26667770
  3. We demonstrated that Mrad9 null enhances chromatid aberration frequency induced by radiation in bystander mouse embryonic stem cells PMID: 25234738
  4. RAD9A is essential for male fertility and for repair of DNA double-strand breaks during meiotic prophase I. PMID: 23788429
  5. A review of the many activities assigned to Rad9, and speculation as to which influence its function in tumor development. PMID: 22034047
  6. Data show that Rad9 plays dual roles in generating functional antibodies and in maintaining the integrity of the whole genome in B cells. PMID: 20729201
  7. Rad9A-mediated Claspin localization is a vital step during checkpoint activation. PMID: 20081369
  8. tousled-like kinases play important roles in DNA repair, not only by modulation of chromatin assembly via Asf1, but also by a more direct function in processing the ends of a tousled-like kinase via interaction with Rad9. PMID: 20021694
  9. HRAD9 and Mrad9 are part of a gene family and reveal a new genetic element encoding a product that interacts with multiple, known cell cycle checkpoint control proteins. PMID: 14500360
  10. Results establish mouse rad9 as a key mammalian genetic element of pathways that regulate the cellular response to DNA damage, maintenance of genomic integrity, and proper embryonic development. PMID: 15282322
  11. The encoded mammalian proteins participate in promoting resistance to DNA damage, cell cycle checkpoint control, DNA repair, and apoptosis. PMID: 16365875
  12. Rad9 levels are high in prostate cancer cells due at least in part to aberrant methylation or gene amplification PMID: 18316588
  13. Mrad9 plays an important role in maintaining genomic stability and preventing tumor development in keratinocytes PMID: 18632607
  14. These results suggest that phosphorylation of Rad9 plays a critical role in the activation of the S phase/Mitosis checkpoint, and that downstream proteins cdc2 and 14-3-3sigma mediate this function. PMID: 18769057
  15. Rad9 is a signal in the earlier stage of epithelial cell cycle regulation and plays protective roles in alveolar epithelial cells in the pathogenesis of acute lung injury. PMID: 18809378
  16. Disruption of the Rad9-MLH1 interaction by a single-point mutation in Rad9 leads to significantly reduced DNA mismatch repair activity. PMID: 18842633

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Subcellular Location
Nucleus.
Protein Families
Rad9 family
Tissue Specificity
Expressed in heart, brain, spleen, lung, liver, skeletal muscle, kidney and testis.
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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Address
7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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