Recombinant Mouse Cell cycle checkpoint control protein RAD9A (Rad9a)

Code CSB-YP896673MO
MSDS
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Source Yeast
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Code CSB-EP896673MO
MSDS
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Source E.coli
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Code CSB-EP896673MO-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP896673MO
MSDS
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Source Baculovirus
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Code CSB-MP896673MO
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Rad9a
Uniprot No.
Alternative Names
Rad9a; Rad9; Cell cycle checkpoint control protein RAD9A; mRAD9; EC 3.1.11.2; DNA repair exonuclease rad9 homolog A; Rad9-like protein
Species
Mus musculus (Mouse)
Expression Region
1-389
Target Protein Sequence
MKCLITGGNV KVLGKAVHSL SRIGDELYLE PLKDGLSLRT VNSSRSAYAC FLFAPLFFQQ YQAASPGQDL LRCKILMKAF LSVFRSLAIV EKSVEKCCIS LSGSHSHLVV QLHCKYGVKK THNLSFQDCE SLQAVFDPAS CPHLLRTPAR VLAEAVLSFP LALTEVTLGI GRGRRVILRS YQEEEADSTS KAMVTETSIG DEDFQQLHAP EGIAVTFCLK EFRGLLSFAE SANLPLTIHF DVPGRPVIFT IEDSLLDAHF VLATLLEQDS CSQGPCSPKP HQPVPQKQAH STPHLDDFTS DDIDCYMIAM ETTGGNEGSG AQPSTSLPPV SLASHDLAPT SEEEAEPSTV PGTPPPKKFR SLFFGSILAP VHSPQGPNPV LAEDSDGEG
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Component of the 9-1-1 cell-cycle checkpoint response complex that plays a major role in DNA repair. The 9-1-1 complex is recruited to DNA lesion upon damage by the RAD17-replication factor C (RFC) clamp loader complex. Acts then as a sliding clamp platform on DNA for several proteins involved in long-patch base excision repair (LP-BER). The 9-1-1 complex stimulates DNA polymerase beta (POLB) activity by increasing its affinity for the 3'-OH end of the primer-template and stabilizes POLB to those sites where LP-BER proceeds; endonuclease FEN1 cleavage activity on substrates with double, nick, or gap flaps of distinct sequences and lengths; and DNA ligase I (LIG1) on long-patch base excision repair substrates. The 9-1-1 complex is necessary for the recruitment of RHNO1 to sites of double-stranded breaks (DSB) occurring during the S phase. RAD9A possesses 3'->5' double stranded DNA exonuclease activity.
Gene References into Functions
  1. Rad9a is indispensable for spermatogonia differentiation and testicular development in mice. PMID: 27861152
  2. RAD9 has a prominent role in the ATR-Chk1 pathway that is necessary for successful formation of the damage-sensing complex and DNA damage checkpoint signaling. PMID: 26667770
  3. We demonstrated that Mrad9 null enhances chromatid aberration frequency induced by radiation in bystander mouse embryonic stem cells PMID: 25234738
  4. RAD9A is essential for male fertility and for repair of DNA double-strand breaks during meiotic prophase I. PMID: 23788429
  5. A review of the many activities assigned to Rad9, and speculation as to which influence its function in tumor development. PMID: 22034047
  6. Data show that Rad9 plays dual roles in generating functional antibodies and in maintaining the integrity of the whole genome in B cells. PMID: 20729201
  7. Rad9A-mediated Claspin localization is a vital step during checkpoint activation. PMID: 20081369
  8. tousled-like kinases play important roles in DNA repair, not only by modulation of chromatin assembly via Asf1, but also by a more direct function in processing the ends of a tousled-like kinase via interaction with Rad9. PMID: 20021694
  9. HRAD9 and Mrad9 are part of a gene family and reveal a new genetic element encoding a product that interacts with multiple, known cell cycle checkpoint control proteins. PMID: 14500360
  10. Results establish mouse rad9 as a key mammalian genetic element of pathways that regulate the cellular response to DNA damage, maintenance of genomic integrity, and proper embryonic development. PMID: 15282322
  11. The encoded mammalian proteins participate in promoting resistance to DNA damage, cell cycle checkpoint control, DNA repair, and apoptosis. PMID: 16365875
  12. Rad9 levels are high in prostate cancer cells due at least in part to aberrant methylation or gene amplification PMID: 18316588
  13. Mrad9 plays an important role in maintaining genomic stability and preventing tumor development in keratinocytes PMID: 18632607
  14. These results suggest that phosphorylation of Rad9 plays a critical role in the activation of the S phase/Mitosis checkpoint, and that downstream proteins cdc2 and 14-3-3sigma mediate this function. PMID: 18769057
  15. Rad9 is a signal in the earlier stage of epithelial cell cycle regulation and plays protective roles in alveolar epithelial cells in the pathogenesis of acute lung injury. PMID: 18809378
  16. Disruption of the Rad9-MLH1 interaction by a single-point mutation in Rad9 leads to significantly reduced DNA mismatch repair activity. PMID: 18842633

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Subcellular Location
Nucleus.
Protein Families
Rad9 family
Tissue Specificity
Expressed in heart, brain, spleen, lung, liver, skeletal muscle, kidney and testis.
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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