Product Details

Full Product Name

Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) RGL2 Polyclonal antibody

Uniprot No.

Q8GXW1

Target Names

RGL2

Alternative Names

RGL2 antibody; SCL19 antibody; At3g03450 antibody; T21P5.13 antibody; DELLA protein RGL2 antibody; GRAS family protein 15 antibody; AtGRAS-15 antibody; RGA-like protein 2 antibody; Scarecrow-like protein 19 antibody; AtSCL19 antibody

Raised in

Rabbit

Species Reactivity

Arabidopsis thaliana

Immunogen

Recombinant Arabidopsis thaliana RGL2 protein

Immunogen Species

Arabidopsis thaliana (Mouse-ear cress)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Target Background

Function

Probable transcriptional regulator that acts as a repressor of the gibberellin (GA) signaling pathway. No effect of the BOI proteins on its stability. Probably acts by participating in large multiprotein complexes that repress transcription of GA-inducible genes. Upon GA application, it is degraded by the proteasome, allowing the GA signaling pathway. Acts as a major GA-response repressor of seed germination, including seed thermoinhibition. Promotes the biosynthesis of abscisic acid (ABA), especially in seed coats to maintain seed dormancy. Delays flowering and adult leaf production. Also regulates the floral development, petal, stamen and anther development, by repressing the continued growth of floral organs. Its activity is probably regulated by other phytohormones such as auxin and ethylene. Involved in the regulation of seed dormancy and germination, including glucose-induced delay of seed germination. Promotes salt tolerance. Acts as a repressor of positive regulators of trichome initiation. Required during the flagellin-derived peptide flg22-mediated growth inhibition. Contributes to the susceptibility to the biotrophic pathogen P.syringae pv. tomato and to the resistance to the necrotrophic pathogens B.cinerea A.brassicicola, probably by repressing the SA-defense pathway and preventing cell death. Prevents stress-induced reactive oxygen species (ROS) accumulation (e.g. salt stress) by acting on the ROS scavenging system, and delays ROS-induced cell death, thus promoting stress tolerance.

Gene References into Functions

NF-YC-RGL2-ABI5 module integrates gibberellic acid and abscisic acid signalling pathways during seed germination PMID: 27624486
RGL2-containing protein complex can bind the promoter of GATA12 and thereby regulate its expression PMID: 28917589
The data indicate that AtGASA6 links RGL2 and AtEXPA1 functions and plays a role as an integrator of gibberellin, abscisic acid, and glucose signaling, resulting in the regulation of seed germination through a promotion of cell elongation. PMID: 26400990
RGL2 directly regulates several genes affecting various physiological aspects to inhibit germination. PMID: 23035751
The persistent high ABA levels in imbibed dormant seeds requires the permanent expression of the DELLA gene RGL2, where it remains insensitive to gibberellins (GA) unlike in nondormant seeds. PMID: 20956298
We confirm that RGL2, one member of the DELLA family, encodes the predominant repressor of seed germination in Arabidopsis and show that the other DELLA genes GAI,RGA and RGL1 enhance the function of RGL2. PMID: 16034591
role of conserved serine and threonine in GA signaling PMID: 16167898
Four conserved tyrosine residues within the RGL2 protein are critical for gibberellin sensitivity in Arabidopsis. PMID: 17333251
RGL2 disappearance is not a prerequisite for seed germination in the sly1 background. PMID: 17384169
RGL2 inhibits Arabidopsis seed germination by stimulating abscisic acid synthesis PMID: 18941053

Hide All

Subcellular Location

Nucleus.

Protein Families

GRAS family, DELLA subfamily

Tissue Specificity

Predominantly expressed in germinating seeds, flowers and siliques. Only detectable in the inflorescence, with high levels in young flower buds and significant levels in siliques. In imbibed seeds, it is restricted to seed coats, elongating regions of pre

Database Links

KEGG: ath:AT3G03450

STRING: 3702.AT3G03450.1

UniGene: At.18489

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RGL2 Antibody

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