Product Details

Full Product Name

Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) RHM1 Polyclonal antibody

Uniprot No.

Q9SYM5

Target Names

RHM1

Alternative Names

RHM1 antibody; ROL1 antibody; At1g78570 antibody; T30F21.10 antibody; Trifunctional UDP-glucose 4,6-dehydratase/UDP-4-keto-6-deoxy-D-glucose 3,5-epimerase/UDP-4-keto-L-rhamnose-reductase RHM1 antibody; Protein REPRESSOR OF LRX1 1 antibody; Rhamnose biosynthetic enzyme 1 antibody; AtRHM1) [Includes: UDP-glucose 4,6-dehydratase antibody; EC 4.2.1.76); UDP-4-keto-6-deoxy-D-glucose 3,5-epimerase/UDP-4-keto-L-rhamnose 4-keto-reductase antibody; EC 1.1.1.- antibody; EC 5.1.3.-)] antibody

Raised in

Rabbit

Species Reactivity

Arabidopsis thaliana

Immunogen

Recombinant Arabidopsis thaliana RHM1 protein

Immunogen Species

Arabidopsis thaliana (Mouse-ear cress)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Target Background

Function

Trifunctional enzyme involved in UDP-beta-L-rhamnose biosynthesis, a precursor of the primary cell wall components rhamnogalacturonan I (RG-I) and rhamnogalacturonan II (RG-II). Plays a major role in supplying UDP-rhamnose for flavonol biosynthesis. Catalyzes the dehydration of UDP-glucose to form UDP-4-dehydro-6-deoxy-D-glucose followed by the epimerization of the C3' and C5' positions of UDP-4-dehydro-6-deoxy-D-glucose to form UDP-4-keto-beta-L-rhamnose and the reduction of UDP-4-keto-beta-L-rhamnose to yield UDP-beta-L-rhamnose.

Gene References into Functions

Mutations in Arabidopsis RHAMNOSE BIOSYNTHESIS 1 (RHM1) cause dramatic left-handed helical growth of petal epidermal cells, leading to left-handed twisted petals. rhm1 mutant roots also display left-handed growth. PMID: 28736166
GFP::AtRHM1 fusion protein was found to be localized in the cytosol of cotyledon cells and of petiole cells of cotyledon, indicating that AtRHM1 is a cytosol-localized protein. Overexpression resulted in an increase of rhamnose in the leaf cell wall as well as an alteration in galactose and glucose PMID: 19056285
AtRHM1 is induced at transcriptional level by glucose and the regulatory elements involved in the glucose response are located in the region of -931 bp - -752 bp which contains three G-box motifs. PMID: 19160833
In rol1 mutants of Arabidopsis thaliana, flavonols in the cell are glycosylated, rhamnose biosynthesis is affected, flavonol glycosylation profile is modified, and plant development and cell shape are changed. PMID: 18567791
A comparison of flavonol profile in RHM knockout mutants indicated that RHM1 plays a major role in supplying UDP-rhamnose for flavonol modification. PMID: 18757557

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Subcellular Location

Cytoplasm, cytosol.

Protein Families

NAD(P)-dependent epimerase/dehydratase family, dTDP-glucose dehydratase subfamily; DTDP-4-dehydrorhamnose reductase family

Tissue Specificity

Expressed in roots, stems, leaves, seedlings, inflorescence tips, and siliques. Detected in the adaxial side of cotyledons, in the emerging leaves and in trichomes. Also detected in the root tip, more precisely in the epidermal cells in the meristematic a

Database Links

KEGG: ath:AT1G78570

STRING: 3702.AT1G78570.1

UniGene: At.198

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RHM1 Antibody

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