rlmH Antibody

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Code	CSB-PA364174XA01ENV
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Product Details

Full Product Name

Rabbit anti-Escherichia coli (strain K12) rlmH Polyclonal antibody

Uniprot No.

P0A8I8

Target Names

rlmH

Alternative Names

rlmH antibody; ybeA antibody; b0636 antibody; JW0631 antibody; Ribosomal RNA large subunit methyltransferase H antibody; EC 2.1.1.177 antibody; 23S rRNA antibody; pseudouridine1915-N3)-methyltransferase antibody; 23S rRNA m3Psi1915 methyltransferase antibody; rRNA antibody; pseudouridine-N3-)-methyltransferase RlmH antibody

Raised in

Rabbit

Species Reactivity

Escherichia coli (strain K12)

Immunogen

Recombinant Escherichia coli (strain K12) rlmH protein

Immunogen Species

Escherichia coli (strain K12)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Function

Specifically methylates the pseudouridine at position 1915 (m3Psi1915) in 23S rRNA. Specific for fully assembled 70S ribosomes.

Gene References into Functions

Data suggest that E. coli RlmH functions as a dimer, with the S-adenosyl-methionine binding pocket of one monomer and the C-terminal residues of its counterpart forming a composite active site. Study proposes that the C-terminal tail provides a residue that acts as a general base while the other conserved residues form a network of interactions that organize the catalytic site. PMID: 28428565
While the synthesis of Psis in H69 by RluD is relatively insensitive to the point mutations at neighboring positions, methylation of one of the Psis by RlmH exhibited a much stronger sensitivity. PMID: 22586702
show that RlmH has unique substrate specificity among rRNA modification enzymes PMID: 20817755
The identity of the methyltransferase YbeA (redesignated RlmH for rRNA large subunit methyltransferase H) that specifically catalyzes methyl group addition to form m(3)Psi1915 is reported. PMID: 18755835
E. coli protein YbeA as the methyltransferase methylating Psi1915 in 23S rRNA was identified. PMID: 18755836

Hide All

Subcellular Location

Cytoplasm.

Protein Families

RNA methyltransferase RlmH family

Database Links

KEGG: ecj:JW0631

STRING: 316385.ECDH10B_0705

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Service

Phage Display Service

Antibody Service

Protein Service

Gene Synthesis Service

Oligo Synthesis Service

Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

Call us

301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

[email protected]

Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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rlmH Antibody

Product Details

Related Products

Customer Reviews and Q&A

Target Background