Product Details

Full Product Name

Rabbit anti-Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) sap1 Polyclonal antibody

Uniprot No.

P40847

Target Names

sap1

Alternative Names

sap1 antibody; SPCC1672.02c antibody; Switch-activating protein 1 antibody

Raised in

Rabbit

Species Reactivity

Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)

Immunogen

Recombinant Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) sap1 protein

Immunogen Species

Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Target Background

Function

Binds to sequences required for mating-type switching. Makes a simultaneous contact with both the alpha and beta domains of the switch-activating site SAS1. Also binds to replication fork barrier 1 (RFB1) located within a 78 base pair sequence near the 3' end of the rRNA coding region. This leads to replication fork blockage. It binds the consensus sequence 5'-TA[AG]GCAGNTN[CT]AACG[AC]G-3'.; Has a role in chromosome organization and integrity where it is involved in chromosome segregation. Has a role in sister chromatid cohesion and condensation.

Gene References into Functions

Sap1 is a replication-initiation factor that directly participates in the assembly of the pre-RC. DNA-replication origins in fission yeast are defined by possessing two essential elements with one bound by ORC and the other by Sap1. PMID: 28223353
This study shows that insertion of the fission yeast LTR retrotransposon Tf1 is guided by the DNA binding protein Sap1 and that the efficiency and location of the targeting depend on the activity of Sap1 as a replication fork barrier. PMID: 26404838
Sap1p binds the rDNA fork barrier Ter1 to cause polar replication fork arrest at this site but not at SAS1. PMID: 16195226
Results reveal that Sap1-DNA affinity alone is insufficient to account for fork arrest and suggest that Sap1 binding-induced structural changes may result in formation of a competent fork-blocking complex. PMID: 16940176
Sap1 is required to recruit the replication fork protection complex to chromatin, thereby contributing to the activation of the replication checkpoint and the stabilization of replication forks. PMID: 17151242
Sap1 is required for replication fork arrest at the rRNA genes of fission yeast PMID: 16166653

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Subcellular Location

Nucleus.

Database Links

KEGG: spo:SPCC1672.02c

STRING: 4896.SPCC1672.02c.1

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sap1 Antibody

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