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| Code | CSB-PA342557XA01SVG |
| Size | US$299 |
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The production of polyclonal antibodies targeting SEC16 necessitates repetitive immunizations of a rabbit with recombinant Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SEC16 protein (280-430aa). Once an adequate antibody titer is achieved, the rabbit is bled, and the serum-derived antibodies are purified via affinity chromatography. Subsequent evaluation of the resulting SEC16 antibody's functionality occurs through ELISA and WB assays, affirming its ability to interact with Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SEC16 protein.
The Saccharomyces cerevisiae SEC16 is a crucial player in the early stages of the secretory pathway. Operating at endoplasmic reticulum exit sites (ERES), SEC16 serves as a scaffold protein, organizing and maintaining functional ERES, which are vital for vesicle formation during protein transport. Additionally, SEC16 is implicated in cellular responses to stress conditions affecting the ER, highlighting its role in maintaining cellular homeostasis under challenging circumstances.
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KEGG: sce:YPL085W
STRING: 4932.YPL085W
