Product Details

Full Product Name

Rabbit anti-Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SGF73 Polyclonal antibody

Uniprot No.

P53165

Target Names

SGF73

Alternative Names

SGF73 antibody; YGL066W antibody; SAGA-associated factor 73 antibody; 73 kDa SAGA-associated factor antibody; SAGA histone acetyltransferase complex 73 kDa subunit antibody

Raised in

Rabbit

Species Reactivity

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Immunogen

Recombinant Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SGF73 protein

Immunogen Species

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Target Background

Function

Functions as component of the transcription regulatory histone acetylation (HAT) complex SAGA. SAGA is involved in RNA polymerase II-dependent transcriptional regulation of approximately 10% of yeast genes. At the promoters, SAGA is required for recruitment of the basal transcription machinery. It influences RNA polymerase II transcriptional activity through different activities such as TBP interaction (SPT3, SPT8 and SPT20) and promoter selectivity, interaction with transcription activators (GCN5, ADA2, ADA3 and TRA1), and chromatin modification through histone acetylation (GCN5) and deubiquitination (UBP8). SAGA acetylates nucleosomal histone H3 to some extent (to form H3K9ac, H3K14ac, H3K18ac and H3K23ac). SAGA interacts with DNA via upstream activating sequences (UASs).

Gene References into Functions

The replicative lifespan-extending deletion of SGF73 results in altered ribosomal gene expression. PMID: 28568901
The 3D structure of SAGA complexes in which the subunit Sgf73 is partially or fully deleted to remove the deubiquitination module revealed the module localized in the vicinity of Gcn5 and Spt7. PMID: 25441028
The Sgf73 subunit of the SAGA deubiquitinating module (DUBm) activates the catalytic Ubp8 subunit. PMID: 25526805
Sgf73 region (373-402 aa) is essential for heterochromatin boundary function. PMID: 23819448
Solution NMR and circular dichroism analysis of Sgf73(1-104) after zinc ion removal using chelation reagent EDTA demonstrated that zinc ion was required to maintain stable conformation of the zinc finger motif. PMID: 20510875
study reports the 1.90 angstrom resolution crystal structure of the deubiquitinating module (DUBm)- which contains Ubp8 bound to Sgf11, Sus1 and Sgf73- bound to ubiquitin aldehyde, as well as the 2.45 angstrom resolution structure of the uncomplexed DUBm PMID: 20395473

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Subcellular Location

Nucleus.

Protein Families

Ataxin-7 family

Database Links

KEGG: sce:YGL066W

STRING: 4932.YGL066W

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SGF73 Antibody

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