alpha-Spec Antibody

Code CSB-PA022632XA01DLU
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Product Details

Full Product Name
Rabbit anti-Drosophila melanogaster (Fruit fly) alpha-Spec Polyclonal antibody
Uniprot No.
Target Names
alpha-Spec
Alternative Names
alpha-Spec antibody; SPEC-A antibody; CG1977 antibody; Spectrin alpha chain antibody
Raised in
Rabbit
Species Reactivity
Drosophila melanogaster (Fruit fly)
Immunogen
Recombinant Drosophila melanogaster (Fruit fly) alpha-Spec protein
Immunogen Species
Drosophila melanogaster (Fruit fly)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Spectrin is the major constituent of the cytoskeletal network underlying the erythrocyte plasma membrane. It associates with band 4.1 and actin to form the cytoskeletal superstructure of the erythrocyte plasma membrane. Essential for larval survival and development. Stabilizes cell to cell interactions that are critical for the maintenance of cell shape and subcellular organization within embryonic tissues. Lva and spectrin may form a Golgi-based scaffold that mediates interaction of Golgi bodies with microtubules and facilitates Golgi-derived membrane secretion required for the formation of furrows during cellularization.
Gene References into Functions
  1. a functional spectrin-integrin complex is important for regulation of the actomyosin cytoskeleton and tissue architecture PMID: 26952981
  2. Alpha-pectrin regulates Hippo signaling by modulating cytoskeletal actomyosin activity. PMID: 25826608
  3. Our data provide the first rigorous test for the general requirement for tetramer-based non-erythroid spectrin networks throughout an organism and find that they have very limited roles, in direct contrast to the current paradigm PMID: 25381248
  4. The well-characterized interaction between AE1 and the spectrin cytoskeleton in erythrocytes is not conserved in Drosophila. PMID: 20573195
  5. Specific regions of the spectrin molecule were tested for a role in polarized assembly and function. PMID: 18287096
  6. Molecular mechanisms that enhance synaptic stability despite persistent disruption of the spectrin/Ank/microtubule cytoskeleton are reported. PMID: 19805631

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Subcellular Location
Cytoplasm, cytoskeleton. Golgi apparatus. Note=Near the inner surface of the plasma membrane of nearly all cells. Lva-alpha-spectrin complexes are found at the Golgi.
Protein Families
Spectrin family
Tissue Specificity
A substantial pool of maternal protein in the egg undergoes dynamic changes in distribution early in embryogenesis. In gastrulated embryo, the highest level of protein is found in the respiratory tract cells and the lowest in parts of the forming gut.
Database Links

KEGG: dme:Dmel_CG1977

STRING: 7227.FBpp0305100

UniGene: Dm.7397

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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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