Product Details

Full Product Name

Rabbit anti-Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SSK1 Polyclonal antibody

Uniprot No.

Q07084

Target Names

SSK1

Alternative Names

SSK1 antibody; YLR006C antibody; Osmolarity two-component system protein SSK1 antibody

Raised in

Rabbit

Species Reactivity

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Immunogen

Recombinant Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SSK1 protein

Immunogen Species

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Target Background

Function

Final receptor of the SLN1-YPD1-SSK1 two-component regulatory system, which controls activity of the HOG1 pathway in response to changes in the osmolarity of the extracellular environment. Under normal osmotic conditions, maintained in a phosphorylated and inactive state by the phosphorelay intermediate protein YPD1. Under conditions of high osmolarity, the histidine kinase SLN1 is no longer active and the unphosphorylated form of SSK1 interacts with and activates SSK2 and SSK22, two MAPKKKs that further stimulate the PBS2-HOG1 MAPKK-MAPK cascade. Unphosphorylated SSK1 is subsequently degraded by the UBC7-dependent ubiquitin-proteasome system to down-regulate the HOG1 pathway after completion of the osmotic adaptation.

Gene References into Functions

Delayed turnover of unphosphorylated Ssk1 during carbon stress activates the yeast Hog1 map kinase pathway. PMID: 26340004
Our data suggest that Snf1 downregulates ER stress response signal mediated by Hog1 through negatively regulating expression of its specific activator Ssk1 at the transcriptional level PMID: 26394309
network structure of the Sln1-Ypd1-Ssk1 three-component phospho-relay prevents unintended activation of the HOG MAPK pathway in Saccharomyces cerevisiae PMID: 25888817
The osmotic stress response through the Ssk1p-independent Ssk2p activation is strong, although its duration is short compared with the Ssk1p-dependent activation. PMID: 23457455
Ssk1 is required for Hog1 metabolic activation. PMID: 22882253
Constitutive activation of Ssk2 occurs when Ssk1 phosphorylation is blocked by either an Ssk1 mutation at the phosphorylation site or an Ssk1 mutation that inhibits its interaction with Ypd1, the donor of phosphate to Ssk1. PMID: 18573873
Osmolytes negatively affect the stability of the SSK1-3YPD1 complex in a concentration-dependent manner and, as a consequence, the rate of phosphate hydrolysis increases. PMID: 19618914

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Subcellular Location

Cytoplasm.

Database Links

KEGG: sce:YLR006C

STRING: 4932.YLR006C

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SSK1 Antibody

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