TfIIA-S Antibody

Code CSB-PA010000XA01DLU
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Product Details

Full Product Name
Rabbit anti-Drosophila melanogaster (Fruit fly) TfIIA-S Polyclonal antibody
Uniprot No.
Target Names
TfIIA-S
Alternative Names
TfIIA-S antibody; CG5163 antibody; Transcription initiation factor IIA subunit 2 antibody; General transcription factor IIA subunit 2 antibody; TFIIA p14 subunit antibody; TFIIA-14 antibody; Transcription initiation factor IIA gamma chain antibody; TFIIA-gamma antibody; dTFIIA-S antibody
Raised in
Rabbit
Species Reactivity
Drosophila melanogaster (Fruit fly)
Immunogen
Recombinant Drosophila melanogaster (Fruit fly) TfIIA-S protein
Immunogen Species
Drosophila melanogaster (Fruit fly)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
TFIIA is a component of the transcription machinery of RNA polymerase II and plays an important role in transcriptional activation. TFIIA in a complex with TBP mediates transcriptional activity. Part of a rhi-dependent transcription machinery that enables the generation of piRNA precursors from heterochromatin while maintaining the suppression of transposon-encoded promoters and enhancers. Forms a complex with Moonshiner/CG12721 and Trf2 which recruit transcriptional machinery to heterochromatin to initiate the bidirectional transcription of piRNA clusters, by interacting with the RDC (rhi, del and cuff) complex that binds to repressive H3K9me3 marks in the chromatin. This mechanism allows transcription to occur in piRNA clusters despite the lack of proper promoter elements and in the presence of the repressive H3K9me3 mark.
Subcellular Location
Nucleus.
Protein Families
TFIIA subunit 2 family
Tissue Specificity
Ubiquitous.
Database Links

KEGG: dme:Dmel_CG5163

STRING: 7227.FBpp0083912

UniGene: Dm.20591

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