tie2 Antibody

Code CSB-PA524227XA01DIL
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Product Details

Full Product Name
Rabbit anti-Danio rerio (Zebrafish) (Brachydanio rerio) tie2 Polyclonal antibody
Uniprot No.
Target Names
tie2
Alternative Names
tie2 antibody; tie-2 antibody; Tyrosine-protein kinase receptor Tie-2 antibody; EC 2.7.10.1 antibody; Tyrosine kinase with Ig and EGF homology domains-2 antibody
Raised in
Rabbit
Species Reactivity
Danio rerio (Zebrafish) (Brachydanio rerio)
Immunogen
Recombinant Danio rerio (Zebrafish) (Brachydanio rerio) tie2 protein
Immunogen Species
Danio rerio (Zebrafish) (Brachydanio rerio)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Tyrosine-protein kinase that acts as cell-surface receptor for angiopoietins and regulates angiogenesis, endothelial cell survival, proliferation, migration, adhesion and cell spreading, reorganization of the actin cytoskeleton, but also maintenance of vascular quiescence. Can activate or inhibit angiogenesis, depending on the context. Angiopoietin signaling triggers receptor dimerization and autophosphorylation at specific tyrosine residues that then serve as binding sites for scaffold proteins and effectors.
Gene References into Functions
  1. observations demonstrate that Tie2 is an important regulator of tip cell behaviors PMID: 25001479
Subcellular Location
Cell membrane; Single-pass type I membrane protein. Cell junction. Cell junction, focal adhesion. Cytoplasm, cytoskeleton.
Protein Families
Protein kinase superfamily, Tyr protein kinase family, Tie subfamily
Tissue Specificity
Expressed in most populations of endothelial cells in 24 hours embryos. Not present in intersegmental vessels.
Database Links
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Call us
301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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Address
7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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