TIP2-1 Antibody

Code CSB-PA671454XA01DOA
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Product Details

Full Product Name
Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) TIP2-1 Polyclonal antibody
Uniprot No.
Target Names
TIP2-1
Alternative Names
TIP2-1; At3g16240; MYA6.10; Aquaporin TIP2-1; Delta-tonoplast intrinsic protein; Delta-TIP; Tonoplast intrinsic protein 2-1; AtTIP2;1
Raised in
Rabbit
Species Reactivity
Arabidopsis thaliana
Immunogen
Recombinant Arabidopsis thaliana TIP2-1 protein
Immunogen Species
Arabidopsis thaliana (Mouse-ear cress)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Aquaporin required to facilitate the transport of water from the vacuolar compartment to the cytoplasm. Does not promote glycerol permability. Its function is impaired by Hg(2+). Transports urea in yeast cells and Xenopus laevis oocytes in a pH-independent manner. Transports methylammonium or ammonium in yeast cells and Xenopus laevis oocytes, preferentially at high medium pH. May participate in vacuolar compartmentation and detoxification of ammonium.
Gene References into Functions
  1. The relatively wide pore and the polar nature of the selectivity filter clarify the ammonia permeability. PMID: 27028365
  2. AtTIP2;1 and AtTIP2;3 can mediate the extracytosolic transport of methyl-NH2 and NH3 across the tonoplast membrane and may thus participate in vacuolar ammonium compartmentation. [TIP2;1] PMID: 15665250
Subcellular Location
Vacuole membrane; Multi-pass membrane protein.
Protein Families
MIP/aquaporin (TC 1.A.8) family, TIP (TC 1.A.8.10) subfamily
Tissue Specificity
Strongly expressed in shoot, rosette, bolt and flowers. Also expressed in roots, flower buds and above ground.
Database Links

KEGG: ath:AT3G16240

STRING: 3702.AT3G16240.1

UniGene: At.23571

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