Name: TJP1 Antibody
Brand: CUSABIO
SKU: CSB-PA023573ZA01DO

Product Details

Full Product Name	Rabbit anti-Canis lupus familiaris (Dog) (Canis familiaris) TJP1 Polyclonal antibody
Uniprot No.	O97758
Target Names	TJP1
Alternative Names	TJP1 antibody; ZO1Tight junction protein ZO-1 antibody; Tight junction protein 1 antibody; Zona occludens protein 1 antibody; Zonula occludens protein 1 antibody
Raised in	Rabbit
Species Reactivity	Canis lupus familiaris
Immunogen	Recombinant Canis lupus familiaris TJP1 protein
Immunogen Species	Canis lupus familiaris (Dog) (Canis familiaris)
Conjugate	Non-conjugated
Clonality	Polyclonal
Isotype	IgG
Purification Method	Protein A/G
Concentration	It differs from different batches. Please contact us to confirm it.
Buffer	Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form	Liquid
Tested Applications	ELISA, WB (ensure identification of antigen)
Protocols	ELISA Protocol
Troubleshooting and FAQs	Antibody FAQs
Storage	Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables	① 200ug * antigen (positive control); ② 1ml * Pre-immune serum (negative control);
Quality Guarantee	① Antibody purity can be guaranteed above 90% by SDS-PAGE detection; ② ELISA titer can be guaranteed 1: 64,000; ③ WB validation with antigen can be guaranteed positive;
Lead Time	Made-to-order (12-14 weeks)

Target Background

Function (From Uniprot)	TJP1, TJP2, and TJP3 are closely related scaffolding proteins that link tight junction (TJ) transmembrane proteins such as claudins, junctional adhesion molecules, and occludin to the actin cytoskeleton. The tight junction acts to limit movement of substances through the paracellular space and as a boundary between the compositionally distinct apical and basolateral plasma membrane domains of epithelial and endothelial cells. Necessary for lumenogenesis, and particularly efficient epithelial polarization and barrier formation. Plays a role in the regulation of cell migration by targeting CDC42BPBb to the leading edge of migrating cells. With TJP2 and TJP3, participates in the junctional retention and stability of the transcription factor DBPA, but is not involved in its shuttling to the nucleus.
Gene References into Functions	In the present study, organspecific expression of the tight junction proteins, claudin, occludin, junction adhesion molecule A and zona occludens 1 was examined in the canine duodenum, lung, liver and kidney. PMID: 27600198 Occludin and ZO-1 expression remained unchanged between atopic and clinically normal dogs. PMID: 25673908 ZO-1 knockout induced striking changes in myosin organization at cell-cell contacts and disrupted the localization of tight junction proteins. PMID: 25157572 Akt-dependent phosphorylation of Serine 373 increases gap junction size and communication by completely eliminating the interaction between Cx43 and ZO-1. PMID: 24213533 our study identifies alpha-catenin binding to ZO-1 as a new mechanism for coupling the assembly of the epithelial barrier to cell-to-cell adhesion. PMID: 23813953 ZO-1 and ZO-2 proteins are required not only for TJ assembly but also for regulating the organization and functional activity of the apical cytoskeleton, particularly the perijunctional actomyosin ring; these activities are relevant both to cellular organization and epithelial morphogenesis. PMID: 22190737 CPEB-mediated zonal occludens-1 mRNA localization is essential for tight-junction assembly and mammary epithelial cell polarity PMID: 22334078 ZO-1 and PLEKHA7 are paracingulin-interacting proteins that are involved in its recruitment to epithelial tight and adherens junctions, respectively PMID: 21454477 The results indicate that the transient interaction of afadin with ZO-1 is necessary for the formation of Tight junctions in MDCK cells. PMID: 20008323 Apg-2 regulates ZONAB function by competing for binding to the SH3 domain of ZO-1 and suggest that Apg-2 functions as a regulator of ZO-1-ZONAB signaling in epithelial cells in response to cellular stress. PMID: 16407410 These data reveal an unexpected function for the SH3 domain of ZO-1 in regulating tight junction assembly in epithelial cells. PMID: 16436508 a unique motif in the occludin sequence that is involved in the regulation of ZO-1 binding by reversible phosphorylation of specific Tyr residues. PMID: 19017651 Results provide the first direct evidence that ZO-1 limits solute permeability in established tight junctions, perhaps by forming a stabilizing link between the barrier and perijunctional actomyosin. PMID: 19605556 Show More Hide All
Subcellular Location	Cell membrane; Peripheral membrane protein; Cytoplasmic side. Cell junction, tight junction. Cell junction. Cell junction, gap junction.
Protein Families	MAGUK family
Database Links	KEGG: cfa:403752 STRING: 9615.ENSCAFP00000042731 UniGene: Cfa.3616

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TJP1 Antibody

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