Product Details

Full Product Name

Rabbit anti-Zaire ebolavirus (strain Kikwit-95) (ZEBOV) (Zaire Ebola virus) VP30 Polyclonal antibody

Uniprot No.

Q77DJ5

Target Names

VP30

Alternative Names

VP30 antibody; Minor nucleoprotein VP30 antibody; EbolaVP30 antibody; eVP30 antibody; Transcription activator VP30 antibody

Raised in

Rabbit

Species Reactivity

Zaire ebolavirus

Immunogen

Recombinant Zaire ebolavirus Minor nucleoprotein VP30 (VP30) (1-288AA)

Immunogen Species

Zaire ebolavirus (strain Kikwit-95) (ZEBOV) (Zaire Ebola virus)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order

Related Products

Target Background

Function

Multifunctional protein that acts as a viral transcriptional activator. Promotes read-through of an RNA hairpin in the NP open reading frame to enhance viral transcription. Mechanistically, nonphosphorylated VP30 hexamers form a ternary complex with the viral leader RNA. Clamps the RNA template and the complex VP35-polymerase L together, thereby increasing the polymerase affinity for the RNA template to increase transcription initiation despite the presence of RNA secondary structures. Assists also stop-start transcription at gene junctions to promote transcription of downstream genes. Interaction with NP plays a critical role in transcription initiation by recognizing the RNA stem loop. Interaction with host RBBP6 interferes with NP-VP30 interaction and inhibits viral RNA synthesis. Also acts as a suppressor of RNA silencing by interacting with host DICER1 and TARBP2/TRBP.

Gene References into Functions

Functional analyses of the eVP30-nucleoprotein interface identify residues that are critical for viral RNA synthesis. Altogether, these results support a model where the eVP30-nucleoprotein interaction plays a critical role in transcription initiation and provides a novel target for the development of antiviral therapy. PMID: 28593988
Comparison of the percentage of disorder (PID) in nucleocapsid proteins VP30 and NP reveals high correlation between nucleocapsid PIDs and the case-fatality rates of Ebola virus . PMID: 26086270
mutations of Thr(143) and Thr(146) of VP30 significantly inhibited EBOV transcription and strongly induced VP30 phosphorylation in the N-terminal Ser residues 29-46, suggesting a novel mechanism of regulation of VP30 phosphorylation. PMID: 24936058
Study shows for the first time the direct interaction of EBOV VP30 with RNA and the importance of the N-terminal region for binding RNA. PMID: 17567691
VP30 is involved in reinitiation of gene transcription and that this activity is affected by mutations at the phosphorylation sites. PMID: 18829754

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Subcellular Location

Virion. Host cytoplasm.

Protein Families

Filoviridae minor nucleoprotein VP30 family

Database Links

KEGG: vg:911826

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VP30 Antibody

Product Details

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Target Background