alkB Antibody

Code CSB-PA489400ZA01DQP
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Product Details

Full Product Name
Rabbit anti-Caulobacter vibrioides (strain NA1000 / CB15N) (Caulobacter crescentus) alkB Polyclonal antibody
Uniprot No.
Target Names
alkB
Alternative Names
Alpha-ketoglutarate-dependent dioxygenase AlkB homolog (EC 1.14.11.33) (DNA oxidative demethylase AlkB), alkB
Raised in
Rabbit
Species Reactivity
Caulobacter vibrioides
Immunogen
Recombinant Caulobacter vibrioides alkB protein
Immunogen Species
Caulobacter vibrioides (strain NA1000 / CB15N) (Caulobacter crescentus)
Conjugate
Non-conjugate
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (12-14 weeks)
Usage
For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Function
Dioxygenase that repairs alkylated DNA and RNA containing 3-methylcytosine or 1-methyladenine by oxidative demethylation. Has highest activity towards 3-methylcytosine. Has lower activity towards alkylated DNA containing ethenoadenine, and no detectable activity towards 1-methylguanine or 3-methylthymine. Accepts double-stranded and single-stranded substrates. Requires molecular oxygen, alpha-ketoglutarate and iron. Provides extensive resistance to alkylating agents such as MMS and DMS (SN2 agents), but not to MMNG and MNU (SN1 agents).
Protein Families
AlkB family
Database Links
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Address
7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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