dltA Antibody

Code CSB-PA336594ZA01BRJ
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Product Details

Full Product Name
Rabbit anti-Bacillus subtilis (strain 168) dltA Polyclonal antibody
Uniprot No.
Target Names
dltA
Alternative Names
dltA antibody; dae antibody; BSU38500 antibody; ipa-5rD-alanine--D-alanyl carrier protein ligase antibody; DCL antibody; EC 6.2.1.54 antibody; D-alanine--poly(phosphoribitol) ligase subunit 1 antibody; D-alanine-activating enzyme antibody; DAE antibody
Raised in
Rabbit
Species Reactivity
Bacillus subtilis
Immunogen
Recombinant Bacillus subtilis dltA protein
Immunogen Species
Bacillus subtilis (strain 168)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (12-14 weeks)

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Target Background

Function
Catalyzes the first step in the D-alanylation of lipoteichoic acid (LTA), the activation of D-alanine and its transfer onto the D-alanyl carrier protein (Dcp) DltC. In an ATP-dependent two-step reaction, forms a high energy D-alanyl-AMP intermediate, followed by transfer of the D-alanyl residue as a thiol ester to the phosphopantheinyl prosthetic group of the Dcp. D-alanylation of LTA plays an important role in modulating the properties of the cell wall in Gram-positive bacteria, influencing the net charge of the cell wall.
Gene References into Functions
  1. DltA synthesizes not only N-(d-alanyl)-l-cysteine (a dipeptide) but also various oligopeptides. PMID: 26586916
  2. assayed the substrate selectivity of DltA with its substrate DltC PMID: 15955059
  3. analysis of the crystal structure of DltA PMID: 18784082
Subcellular Location
Cytoplasm.
Protein Families
ATP-dependent AMP-binding enzyme family, DltA subfamily
Database Links
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Call us
301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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Address
7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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