inhA Antibody

Code CSB-PA363781ZA01MVZ
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Product Details

Full Product Name
Rabbit anti-Mycobacterium tuberculosis (strain CDC 1551 / Oshkosh) inhA Polyclonal antibody
Uniprot No.
Target Names
inhA
Alternative Names
inhA antibody; MT1531 antibody; Enoyl-[acyl-carrier-protein] reductase [NADH] antibody; ENR antibody; Enoyl-ACP reductase antibody; EC 1.3.1.9 antibody; FAS-II enoyl-ACP reductase antibody; NADH-dependent 2-trans-enoyl-ACP reductase antibody
Raised in
Rabbit
Species Reactivity
Mycobacterium tuberculosis
Immunogen
Recombinant Mycobacterium tuberculosis inhA protein
Immunogen Species
Mycobacterium tuberculosis (strain CDC 1551 / Oshkosh)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (12-14 weeks)

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Target Background

Function
Enoyl-ACP reductase of the type II fatty acid syntase (FAS-II) system, which is involved in the biosynthesis of mycolic acids, a major component of mycobacterial cell walls. Catalyzes the NADH-dependent reduction of the double bond of 2-trans-enoyl-[acyl-carrier protein], an essential step in the fatty acid elongation cycle of the FAS-II pathway. Shows preference for long-chain fatty acyl thioester substrates (>C16), and can also use 2-trans-enoyl-CoAs as alternative substrates. The mycobacterial FAS-II system utilizes the products of the FAS-I system as primers to extend fatty acyl chain lengths up to C56, forming the meromycolate chain that serves as the precursor for final mycolic acids.; Is the primary target of the first-line antitubercular drug isoniazid (INH) and of the second-line drug ethionamide (ETH). Overexpressed inhA confers INH and ETH resistance to M.tuberculosis. The mechanism of isoniazid action against InhA is covalent attachment of the activated form of the drug to the nicotinamide ring of NAD and binding of the INH-NAD adduct to the active site of InhA. Similarly, the ETH-NAD adduct binds InhA.
Protein Families
Short-chain dehydrogenases/reductases (SDR) family, FabI subfamily
Database Links

KEGG: mtc:MT1531

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