stcE Antibody

Code CSB-PA530572ZA01EOD
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Product Details

Full Product Name
Rabbit anti-Escherichia coli O157:H7 stcE Polyclonal antibody
Uniprot No.
Target Names
stcE
Alternative Names
Metalloprotease StcE (EC 3.4.24.-) (Mucinase) (Neutral zinc metalloprotease StcE) (Secreted protease of C1 esterase inhibitor from EHEC), stcE, tagA
Raised in
Rabbit
Species Reactivity
Escherichia coli O157:H7
Immunogen
Recombinant Escherichia coli O157:H7 stcE protein
Immunogen Species
Escherichia coli O157:H7
Conjugate
Non-conjugate
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (12-14 weeks)

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Target Background

Function
Virulence factor that contributes to intimate adherence of enterohemorrhagic E.coli (EHEC) O157:H7 to host cells. Is able to cleave the secreted human mucin 7 (MUC7) and the glycoprotein 340 (DMBT1/GP340). Also cleaves human C1 inhibitor (SERPING1), a regulator of multiple inflammatory pathways, and binds and localizes it to bacterial and host cell surfaces, protecting them from complement-mediated lysis. Therefore, the current model proposes two roles for StcE during infection: it acts first as a mucinase, allowing passage of EHEC through the oral cavity by cleaving the salivary glycoproteins that are responsible for bacterial aggregation. Similarly, in the colon, StcE cleaves the glycoproteins that protect the intestinal epithelial surface, allowing EHEC to come into close contact with host cell membranes. Secondly, it acts as an anti-inflammatory agent by localizing SERPING1 to cell membranes.
Subcellular Location
Secreted. Note=Secreted via the etp type II secretion pathway.
Database Links

KEGG: ece:Z_L7031

STRING: 155864.L7031

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