Bovine Alpha-2-HS-glycoprotein(AHSG) ELISA kit

Instructions
Code CSB-EL001485BO
Size 96T,5×96T,10×96T
See More Details 24T ELISA kits trial application
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Product Details

Target Name alpha-2-HS-glycoprotein
Alternative Names AHSG ELISA Kit; FETUAAlpha-2-HS-glycoprotein ELISA Kit; Asialofetuin ELISA Kit; Fetuin-A ELISA Kit
Abbreviation AHSG
Uniprot No. P12763
Species Bos taurus (Bovine)
Sample Types serum, plasma, tissue homogenates
Detection Range 125 ng/mL-8000 ng/mL
Sensitivity 31.25 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Metabolism
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of bovine AHSG in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:100 Average % 96  
Range % 92-98  
1:200 Average % 97  
Range % 93-102  
1:400 Average % 102  
Range % 98-106  
1:800 Average % 88  
Range % 82-96  
Recovery
The recovery of bovine AHSG spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 95 90-99  
EDTA plasma (n=4) 104 99-108  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average    
8000 0.073 0.072 0.073    
4000 0.138 0.136 0.137    
2000 0.255 0.242 0.249    
1000 0.435 0.447 0.441    
500 0.675 0.686 0.681    
250 1.038 1.049 1.044    
125 1.347 1.248 1.298    
0 1.593 1.695 1.644    
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 5-7 working days

Target Data

Function Promotes endocytosis, possesses opsonic properties and influences the mineral phase of bone. Suggested to have lymphocyte stimulating properties, lipid binding capability and to bind thyroid hormone.
Gene References into Functions
  1. two glycoproteins, AHSG and HPX, represent a pivotal glycoprotein of the cytoprotective activity for WN1316, showing a concrete evidence for the novel glycan-independent function of serum glycoproteins in neuroprotective drug efficacy. PMID: 29016670
  2. Site-specific analysis of the O-glycosylation of bovine fetuin by electron-transfer dissociation mass spectrometry. PMID: 24907489
  3. These experiments revealed a second mechanism of long-term mineral ion stabilization by the fetuin-A that is complementary to the formation of calciprotein particles. PMID: 21156141
Subcellular Location Secreted
Protein Families Fetuin family
Tissue Specificity Liver and bone.
Database Links

KEGG: bta:280988

STRING: 9913.ENSBTAP00000000673

UniGene: Bt.23250

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