Bovine Alpha-2-HS-glycoprotein(AHSG) ELISA kit

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Product Details

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Target Data

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Product Details

Target Name

alpha-2-HS-glycoprotein

Alternative Names

AHSG ELISA Kit; FETUAAlpha-2-HS-glycoprotein ELISA Kit; Asialofetuin ELISA Kit; Fetuin-A ELISA Kit

Abbreviation

AHSG

Uniprot No.

P12763

Species

Bos taurus (Bovine)

Sample Types

serum, plasma, tissue homogenates

Detection Range

125 ng/mL-8000 ng/mL

Sensitivity

31.25 ng/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Metabolism

Assay Principle

quantitative

Measurement

Sandwich

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of bovine AHSG in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:100	Average %	96
	Range %	92-98
1:200	Average %	97
	Range %	93-102
1:400	Average %	102
	Range %	98-106
1:800	Average %	88
	Range %	82-96

Recovery

The recovery of bovine AHSG spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	90-99
EDTA plasma (n=4)	104	99-108

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average
8000	0.073	0.072	0.073
4000	0.138	0.136	0.137
2000	0.255	0.242	0.249
1000	0.435	0.447	0.441
500	0.675	0.686	0.681
250	1.038	1.049	1.044
125	1.347	1.248	1.298
0	1.593	1.695	1.644

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Lead Time

5-7 working days

Target Data

Function	Promotes endocytosis, possesses opsonic properties and influences the mineral phase of bone. Suggested to have lymphocyte stimulating properties, lipid binding capability and to bind thyroid hormone.
Gene References into Functions	two glycoproteins, AHSG and HPX, represent a pivotal glycoprotein of the cytoprotective activity for WN1316, showing a concrete evidence for the novel glycan-independent function of serum glycoproteins in neuroprotective drug efficacy. PMID: 29016670 Site-specific analysis of the O-glycosylation of bovine fetuin by electron-transfer dissociation mass spectrometry. PMID: 24907489 These experiments revealed a second mechanism of long-term mineral ion stabilization by the fetuin-A that is complementary to the formation of calciprotein particles. PMID: 21156141
Subcellular Location	Secreted
Protein Families	Fetuin family
Tissue Specificity	Liver and bone.
Database Links	KEGG: bta:280988 STRING: 9913.ENSBTAP00000000673 UniGene: Bt.23250

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Check Code：

Code	CSB-EL001485BO
Size	96T,5×96T,10×96T
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Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.